PXD060813-1

PXD060813 is an original dataset announced via ProteomeXchange.

Title	Meru co-ordinates spindle orientation with cell polarity and cell cycle progression
Description	Correct mitotic spindle alignment is essential for tissue architecture and plays an important role in cell fate specification through asymmetric cell division. Spindle tethering factors such as Drosophila Mud (NuMA in mammals) are recruited to the cell cortex and capture astral microtubules, pulling the spindle in the correct orientation. However, how spindle tethering complexes read the cell polarity axis and how spindle attachment is coupled to mitotic progression remains poorly understood. We explore these questions in Drosophila sensory organ precursors (SOPs), which divide asymmetrically to give rise to the epidermal mechanosensory bristles. We show that the scaffold protein Meru, which is recruited to the posterior cortex by the Frizzled/Dishevelled planar cell polarity complex, in turn recruits Mud, linking the spindle tethering and polarity machineries. Furthermore, Cyclin A/Cdk1 associates with Meru at the posterior cortex, promoting the formation of the Mud/Meru/Dsh complex via Meru and Dsh phosphorylation. Thus, Meru couples spindle orientation with cell polarity and provides a cell cycle-dependent cue for spindle tethering.
HostingRepository	PRIDE
AnnounceDate	2025-03-12
AnnouncementXML	Submission_2025-03-12_05:13:30.509.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tania Auchynnikava
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-14 04:02:24	ID requested
⏵ 1	2025-03-12 05:13:30	announced

10.1101/2024.10.01.616065;

submitter keyword: polarity,Meru, Spindle

Nicolas Tapon
contact affiliation	The Francis Crick Institute
contact email	nic.tapon@crick.ac.uk
lab head
Tania Auchynnikava
contact affiliation	The Francis Crick Institute
contact email	auchynt@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060813
     1. Label: PRIDE project
     2. Name: Meru co-ordinates spindle orientation with cell polarity and cell cycle progression