PXD060801-1

PXD060801 is an original dataset announced via ProteomeXchange.

Title	Heat stress induced proteomes in tomato pollen mother cells and microspores
Description	During the reproductive stage, tomatoes require nighttime temperatures below 21°C and daytime temperatures below 32°C for healthy flower development and fruit set. Prolonged exposure to temperatures above the optimal range can cause abnormalities in pollen development, leading to reduced fertility or sterility. This project aims to identify alterations in proteome expression and elucidate heat tolerance mechanisms affecting pollen development under elevated temperature conditions. Two tomato varieties—the heat-tolerant ‘Black Vernissage’ and the heat-sensitive ‘Micro-Tom’—were exposed to three temperature treatments: 26 °C (control), 32 °C, and 37 °C (heat-treated). Flower buds containing meiotic pollen mother cells prior to the tetrad stage and free microspores were harvested, and single cell type samples were collected using laser microdissection. Tandem mass tag (TMT) proteomics was employed to identify differential proteomic responses in pollen mother cells and free microspores between stress-treated and non-treated plants.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_07:32:51.987.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060801
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ted Thannhauser
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	methylthiolated residue; monohydroxylated residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-13 19:49:14	ID requested
⏵ 1	2025-05-07 07:32:52	announced

10.6019/PXD060801;

Thapa P, Guo J, Pradhan K, Thapa D, Madhavarapu S, Zou J, Potts J, Li H, O'Hair J, Wang C, Zhou S, Yang Y, Fish T, Thannhauser TW, Cell-Type-Specific Heat-Induced Changes in the Proteomes of Pollen Mother Cells and Microspores Provide New Insights into Tomato Pollen Production Under Elevated Temperature. Proteomes, 13(2):(2025) [pubmed]

10.3390/proteomes13020013;

submitter keyword: laser Capture Microdissection, pollen heat stress, TMT proteomics,tomato free microspores

Theodore Thannhauser
contact affiliation	USDA-ARS
contact email	tt34@cornell.edu
lab head
Ted Thannhauser
contact affiliation	USDA-ARS
contact email	tt34@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060801

PRIDE project URI

• PRIDE
  1. PXD060801
     1. Label: PRIDE project
     2. Name: Heat stress induced proteomes in tomato pollen mother cells and microspores