PXD060777-1

PXD060777 is an original dataset announced via ProteomeXchange.

Title	Chromosome-level reference genome for the medically important Arabian horned viper (Cerastes gasperettii)
Description	Venomous animals have traditionally been studied from a proteomic (but also transcriptomic) perspective, often overlooking the study of venom from a genomic point of view until recently. The rise of genomics has led to an increase in the number of reference genomes for non-model organisms, including venomous taxa, enabling new questions on venom evolution from a genomic context. Although venomous snakes are the fundamental model system in venom research, the number of high-quality reference genomes in the group remains limited. In this study, we present a high-quality chromosome-level reference genome for the Arabian horned viper (Cerastes gasperettii), a highly venomous snake native to the Arabian Peninsula. Our highly-contiguous genome allowed us to explore macrochromosomal rearrangements within the Viperidae family, as well as across squamate reptile evolution. Furthermore, we identified a total of ten different toxins conforming the venom’s core, in line with our proteomic results. We also compared microsyntenic changes in the main toxin gene clusters with those of other venomous snake species, highlighting the pivotal role of gene duplication and loss in the emergence and diversification of the two main toxin families for Cerastes gasperettii. Using Illumina data, we reconstructed the demographic history and genome-wide diversity of the species, revealing how historical aridity likely drove population expansions. Finally, this study highlights the importance of using long-read sequencing as well as chromosome-level reference genomes to disentangle the origin and diversification of toxin families in venomous species.
HostingRepository	PRIDE
AnnounceDate	2025-02-18
AnnouncementXML	Submission_2025-02-18_07:20:34.603.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Davinia Pla
SpeciesList	scientific name: Cerastes gasperettii; NCBI TaxID: 110202;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-13 07:54:23	ID requested
⏵ 1	2025-02-18 07:20:34	announced

Dataset with its publication pending

submitter keyword: Toxin evolution

microsynteny

genomics

transcriptomics

Prof. Dr. Juan José Calvete Chornet
contact affiliation	Evolutionary and translational venomics Lab, Instituto de Biomedicina de Valencia, CSIC
contact email	jcalvete@ibv.csic.es
lab head
Davinia Pla
contact affiliation	CSIC
contact email	dpla@ibv.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD060777

PRIDE project URI

• PRIDE
  1. PXD060777
     1. Label: PRIDE project
     2. Name: Chromosome-level reference genome for the medically important Arabian horned viper (Cerastes gasperettii)