PXD060757 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | SARS-CoV-2 nsp14 induces the m7G methylation and the sequestration of host RNAs in stress granules Twin-Strep Streptactin pull-down LC-MSMS |
| Description | Viruses grab the host translation machinery to facilitate their replication by disturbing the host translation. SARS-CoV-2 nsp14 was known to inhibit host translation, yet the mechanistic details are still obscure. In this study, we report that nsp14 can elevate stress granules (SG) formation in a N7 methyltransferase activity-dependent manner. ER/Golgi localized nsp14 promotes m7G methylation of host RNAs, which are delivered into stress granules (SG), leading to elevated SG formation. Except for a few methylation-associated proteins, most components of SGs induced by nsp14 can be identified in SGs induced by other stress inducers, like sodium arsenite. nsp14-induced SG formation relies on methyl donors, because altering the amount of methyl donors, like decreasing SAM level with overexpression of Glycine N-methyltransferase (GNMT) or increasing SAM level with the supplement of folic acid, can alter SG formation and affect viral replication. Collectively, we characterized nsp14 as an SG formation regulator involved in viral hijacking of the host translation machinery. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-06 |
| AnnouncementXML | Submission_2026-02-06_07:52:21.503.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Weiyi Huang |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-02-12 23:16:11 | ID requested | |
| ⏵ 1 | 2026-02-06 07:52:22 | announced | |
Publication List
Keyword List
| submitter keyword: LC-MSMS, nsp14,Twin-Strep, stress granules |
Contact List
| Xiaoxue Peng |
|---|
| contact affiliation | School of Medicine, Sun Yat-sen University |
| contact email | pengxx9@mail.sysu.edu.cn |
| lab head | |
| Weiyi Huang |
|---|
| contact affiliation | Sun Yat-sen University: Guangzhou, CN |
| contact email | huangwy97@mail2.sysu.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD060757 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060757
- Label: PRIDE project
- Name: SARS-CoV-2 nsp14 induces the m7G methylation and the sequestration of host RNAs in stress granules Twin-Strep Streptactin pull-down LC-MSMS
