PXD060752-1

PXD060752 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of cumulative antenatal and postnatal environmental exposure in the brain of mice
Description	Environmental exposures across critical developmental windows can significantly influence brain development and contribute to the risk of neurodevelopmental disorders (NDDs). Importantly, emerging clinical evidence suggests that multiple environmental factors during early development result in more pronounced disease phenotypes in offspring. To expand upon this existing notion, we developed a ‘triple-hit’ mouse model to examine the combined effects of maternal social stress, chronic high-fat diet consumption, and early life poly(I:C) exposure on long-term developmental outcomes in offspring. We observed that ‘triple-hit’ male offspring displayed autism-like social deficits and an overall increased susceptibility to NDD-like behavioural alterations in adulthood. Single-cell RNA (scRNA) transcriptomic and bulk proteomic analyses were performed in male triple hit offspring in brain tissue. The results are discussed in the associated publication.
HostingRepository	PRIDE
AnnounceDate	2026-01-19
AnnouncementXML	Submission_2026-01-18_17:46:52.021.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060752
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Graham
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-12 20:50:33	ID requested
⏵ 1	2026-01-18 17:46:53	announced

10.1007/s00011-025-02152-y;

Bucknor MC, Keating BA, Han VX, Gloss BS, Dey P, Aryamanesh N, Marshall LL, Graham ME, Dissanayake R, Lau X, Patel S, Petkova SP, Valtchev P, Gururajan A, Dale RC, Hofer MJ, Cumulative environmental exposures adversely impact social behaviour and are associated with dysregulation of genes and proteins involved in epigenetic, ribosomal, and immune regulation in male mice. Inflamm Res, 75(1):17(2026) [pubmed]

10.6019/PXD060752;

submitter keyword: C57bl/6, brain, neurodevelopmental disorder, development, environmental exposure

Markus Hofer
contact affiliation	School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, NSW, Australia
contact email	markus.hofer@sydney.edu.au
lab head
Mark Graham
contact affiliation	Children's Medical Research Institute
contact email	mgraham@cmri.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD060752

PRIDE project URI

• PRIDE
  1. PXD060752
     1. Label: PRIDE project
     2. Name: Proteome analysis of cumulative antenatal and postnatal environmental exposure in the brain of mice