PXD060719-1

PXD060719 is an original dataset announced via ProteomeXchange.

Title	Deciphering the interplay of aflatoxin B1 and Epstein-Barr virus on the histone landscape in Burkitt Lymphoma cells
Description	Aflatoxin B1 (AFB1), a potent dietary carcinogen, and Epstein-Barr virus (EBV), an oncogenic virus, are both implicated in cancer development, particularly in endemic regions. However, the combined effects of AFB1 exposure and EBV infection on the epigenetic landscape remain poorly understood. This study investigates the impact of AFB1 and EBV on histone post-translational modifications (hPTMs) in Burkitt lymphoma (BL) cells through an integrative approach combining untargeted mass spectrometry-based profiling and a time-lapse experimental design. Our results reveal subtle, yet reproducible and dynamic alterations in histone methylation and acetylation patterns over time, with EBV-infected cells exhibiting distinct epigenetic responses compared to uninfected cells. Specifically, AFB1 exposure induced an increase in H3K27me3 levels in EBV-infected cells, counteracting the initial decrease observed upon EBV infection. Additionally, changes in acetylation patterns of H4 N-tail residues and key regulatory proteins suggest potential disruptions in chromatin accessibility and transcriptional regulation. Correlation and network-based analyses further highlight coordinated epigenetic shifts in response to AFB1, with key hubs emerging within the histone code. Despite subtle differences, no significant divergence in overall hPTM responses was detected between EBV-infected and uninfected cells, emphasizing the complexity of epigenetic regulation under combined exposures. These findings provide valuable insights into the interplay between dietary carcinogens and viral infections, shedding light on potential mechanisms driving BL pathogenesis. Future research should explore locus-specific epigenetic changes and therapeutic interventions targeting hPTMs to mitigate cancer risk associated with AFB1 exposure and EBV infection.
HostingRepository	PRIDE
AnnounceDate	2025-10-23
AnnouncementXML	Submission_2025-10-23_04:18:41.586.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060719
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maarten Dhaenens
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monomethylated residue; crotonylated residue; trimethylated residue; alpha-amino propanoylated residue; phosphorylated residue; N6-succinyl-L-lysine; acetylated residue; dimethylated residue; N6-butanoyl-L-lysine; deamidated residue
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-12 05:17:54	ID requested
⏵ 1	2025-10-23 04:18:42	announced

10.6019/PXD060719;

10.1016/J.ENVINT.2025.109813;

submitter keyword: Human, Epstein-Barr virus, Aflatoxin B1, hPTMs, Burkitt Lymphoma cells, LC-MS/MS

Maarten Dhaenens
contact affiliation	ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, B-9000 Ghent, Belgium
contact email	maarten.dhaenens@ugent.be
lab head
Maarten Dhaenens
contact affiliation	Faculity of Pharmaceutical Biotechnology
contact email	maarten.dhaenens@ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD060719

PRIDE project URI

• PRIDE
  1. PXD060719
     1. Label: PRIDE project
     2. Name: Deciphering the interplay of aflatoxin B1 and Epstein-Barr virus on the histone landscape in Burkitt Lymphoma cells