PXD060716-1

PXD060716 is an original dataset announced via ProteomeXchange.

Title	FLAG-tag based co-immunoprecipitation of the Plasmodium falciparum Pfs230:Pfs48/45 complex
Description	Malaria is a devastating disease, causing over half a million deaths annually mostly amongst young children. Transmission blocking vaccines aim to induce immunity against the sexual stages of the causative Plasmodium parasites, preventing human-to-mosquito transmission. Two promising transmission blocking vaccine antigens, Pfs230 and Pfs48/45, form a complex together on the surface of sexual-stage malaria parasites. We created a transgenic parasite line (iGP2230-tag) where the pfs230 gene is fused to a coding sequence for a C-terminal affinity-tag (linker-3xFLAG-linker-EPEA*). Using FLAG-tag resin, we co-immunoprecipitated the Pfs230-FLAG protein from mature gametocytes to identify components of the Pfs230:Pfs48/45 complex.
HostingRepository	PRIDE
AnnounceDate	2025-10-09
AnnouncementXML	Submission_2025-10-09_02:31:21.939.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dick Zijlmans
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: NEWT:5833;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-12 04:11:54	ID requested
⏵ 1	2025-10-09 02:31:22	announced

Dataset with its publication pending

submitter keyword: Malaria, Pfs48/45, Plasmodium falciparum, Pfs230, LC-MS/MS

Matthijs Jore
contact affiliation	Department of Medical Microbiology, Radboud University Medical Center, The Netherlands
contact email	matthijs.jore@radboudumc.nl
lab head
Dick Zijlmans
contact affiliation	Radboud Universiteit Nijmegen
contact email	d.zijlmans@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD060716

PRIDE project URI

• PRIDE
  1. PXD060716
     1. Label: PRIDE project
     2. Name: FLAG-tag based co-immunoprecipitation of the Plasmodium falciparum Pfs230:Pfs48/45 complex