PXD060698-1

PXD060698 is an original dataset announced via ProteomeXchange.

Title	Predictive chemoproteomics and functional validation reveal Coeae6g mediated insecticide cross-resistance in the malaria vector Anopheles gambiae
Description	This project investigates insecticide resistance mechanisms in Anopheles gambiae using Activity-Based Protein Profiling (ABPP). By targeting serine hydrolases (SHs) with fluorophosphonate (FP) probes and mass spectrometry, we identified COEAE6G as a key enzyme conferring resistance to Pirimiphos-methyl (PM) and cross-resistance to other insecticides. Functional validation, including recombinant protein assays and transgenic mosquito lines, confirmed COEAE6G's role in resistance. This work provides valuable insights into resistance mechanisms, aiding the development of sustainable malaria vector control strategies.
HostingRepository	PRIDE
AnnounceDate	2025-10-17
AnnouncementXML	Submission_2025-10-17_08:20:14.045.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hanafy Ismail
SpeciesList	scientific name: Anopheles gambiae str. PEST; NCBI TaxID: NEWT:180454;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-11 13:18:08	ID requested
⏵ 1	2025-10-17 08:20:14	announced

Dataset with its publication pending

submitter keyword: Resistance, Anopheles gambiae, Pirimiphos-methyl,ABPP

Hanafy M.
contact affiliation	Department of Vector Biology Liverpool School of Tropical Medicine Pembroke Place Liverpool L3 5QA UK
contact email	hanafy.ismail@lstmed.ac.uk
lab head
Hanafy Ismail
contact affiliation	Vector Biology Department, Liverpool School of Tropical Medicine Pembroke Pl, Liverpool, Merseyside L3 5QA
contact email	hanafy.ismail@lstmed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD060698

PRIDE project URI

• PRIDE
  1. PXD060698
     1. Label: PRIDE project
     2. Name: Predictive chemoproteomics and functional validation reveal Coeae6g mediated insecticide cross-resistance in the malaria vector Anopheles gambiae