PXD060679-1

PXD060679 is an original dataset announced via ProteomeXchange.

Title	Global distribution of carrageenan foraging pathways reveals widespread latent traits within the genetic “dark matter” of ruminant intestinal microbiomes
Description	Seaweeds represent a promising source of sustainable, alternative feed for livestock. Despite their increasing popularity in agriculture, the dietary fate of seaweed polysaccharides, such as carrageenan, is not known. Here, we applied metagenome-resolved metaproteomicfunctional microbiome analyses to discover catabolic enzymes from the gastrointestinal tract (GIT) microbiomes of ruminants specific for digestion of carrageenans from red seaweeds, such as Mazzaella japonica. Against the expectations of ruminal fermentation, M. japonica significantly instead increases Bacteroides abundance within the lower GIT, and while bacterial isolates can use carrageenans as a sole carbon source. Carrageenan-active polysaccharide utilization loci (CarPULs) were identified and recombinant enzymes were characterized to provide insights into pathway specialization of divergent CarPULs. Selective enrichment and metagenomic mining revealed carrageenan catabolism is widespread among geographically and taxonomically distinct ruminants, suggesting it is a widely distributed latent trait. These pathways are distinct from marine bacteria, highlighting a complex and ancient evolutionary history in the GIT microbiota of animals.
HostingRepository	PRIDE
AnnounceDate	2026-02-25
AnnouncementXML	Submission_2026-02-25_07:54:22.885.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thea Andersen
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-11 06:50:21	ID requested
⏵ 1	2026-02-25 07:54:23	announced

Dataset with its publication pending

submitter keyword: Metagenomics, metagenome-guided metaproteomics, seaweed, carbohydrate utilization

Phil Pope
contact affiliation	Centre for Microbiome Research, School of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute, Woolloongabba, QLD, Australia
contact email	phil.pope@qut.edu.au
lab head
Thea Andersen
contact affiliation	Norwegian University of Life Sciences
contact email	thea.os.andersen@nmbu.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD060679

PRIDE project URI

• PRIDE
  1. PXD060679
     1. Label: PRIDE project
     2. Name: Global distribution of carrageenan foraging pathways reveals widespread latent traits within the genetic “dark matter” of ruminant intestinal microbiomes