PXD060654-1

PXD060654 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis leaf apoplast proteomes under flg22 induced pattern-triggered immunity
Description	The apoplast is a critical interface in plant-pathogen interactions particularly in the context of pattern-triggered immunity (PTI), which is initiated by recognition of microbe-associated molecular patterns (PAMPs). Our study characterizes the proteomic profile of the Arabidopsis apoplast during PTI induced by flg22, a 22 amino acid bacterial flagellin epitope, to elucidate the output of PTI. Apoplastic washing fluid (AWF) was extracted with minimal cytoplasmic contamination for LC-MS/MS analysis. We observed consistent identification of PTI enriched and depleted peptides across replicates with limited correlation between total protein abundance and transcript abundance. We observed topological bias in peptide recovery of receptor-like kinases with peptides predominantly recovered from their ectodomains. Notably, tetraspanin 8, an exosome marker, was enriched in PTI samples. We additionally confirmed increased concentrations of exosomes during PTI. This study enhances our understanding of the proteomic changes in the apoplast during plant immune responses and lays the groundwork for future investigations into the molecular mechanisms of plant defense under recognition of pathogen patterns.
HostingRepository	PRIDE
AnnounceDate	2025-02-11
AnnouncementXML	Submission_2025-02-11_00:41:59.763.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060654
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hsiao-Chun Chen
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-10 14:52:57	ID requested
⏵ 1	2025-02-11 00:42:00	announced

10.6019/PXD060654;

submitter keyword: Tetraspanin, Extracellular vesicle,Proteomics, Pattern-triggered immunity, flg22,Apoplast

Brian Howard
contact affiliation	Department of Plant Pathology, University of Georgia, Athens, Georgia, USA
contact email	bkvitko@uga.edu
lab head
Hsiao-Chun Chen
contact affiliation	University of Georgia
contact email	hcchen@uga.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060654
     1. Label: PRIDE project
     2. Name: Arabidopsis leaf apoplast proteomes under flg22 induced pattern-triggered immunity