PXD060652-1

PXD060652 is an original dataset announced via ProteomeXchange.

Title	RPA70 interactors in BJ and BJ RAS
Description	Activation of oncogenes in healthy cells leads to a permanent cell cycle arrest termed oncogene-induced senescence (OIS) characterized by increased DNA damage and deep epigenetic resetting. Bypassing this premalignant state promotes tumorigenesis. Here, we show that cells entering OIS accumulate high levels of three-stranded RNA–DNA hybrids named R-loops, posing a threat to DNA replication and repair. OIS escape is characterized by the resolution of this extra load of R-loops specifically in genomic regions marked by irreparable damage in OIS. In these loci, the heterotrimeric RPA complex performs quality control over R-loop processing by RNaseH1, reducing the amount of DNA damage and allowing re-entry into the cell cycle. In OIS, hyperphosphorylation of RPA32 decreases the affinity of RPA for RNaseH1; in turn, RNaseH1 loses processivity toward R-loops, leading to double-strand breaks (DSBs) accumulation. These results point to a molecular mechanism that controls R-loop processing and acts as a genome caretaker.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:40:05.858.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD060652
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eros Di Giorgio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-10 14:20:17	ID requested
⏵ 1	2026-04-26 16:40:06	announced

10.6019/PXD060652;

Cortolezzis Y, Tolotto V, Triboli L, Picco R, Soler MA, Fortuna S, Bettin G, D'Este F, Carlassara E, Magris G, Miller KM, Angelini A, Xodo LE, Di Giorgio E, RPA hyperphosphorylation hinders the resolution of R-loops and G-quadruplex-associated R-loops during RAS-driven senescence. Nucleic Acids Res, 54(7):(2026) [pubmed]

10.1093/nar/gkag331;

submitter keyword: SENESCENCE,RPA70, RNASEH1, R-LOOP

Eros Di Giorgio
contact affiliation	Laboratory of Biochemistry, Department of Medicine, University of Udine, Piazzale M. Kolbe 4, 33100 Udine, Italy
contact email	eros.digiorgio@uniud.it
lab head
Eros Di Giorgio
contact affiliation	University of Udine
contact email	eros.digiorgio@uniud.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD060652

PRIDE project URI

• PRIDE
  1. PXD060652
     1. Label: PRIDE project
     2. Name: RPA70 interactors in BJ and BJ RAS