PXD060623-1
PXD060623 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | SDF2 and SDF2L1 are essential co-factors of DNAJB11 for Polycystin-1 processing |
| Description | Mutations in the ER-resident chaperone DNAJB11 have been shown to cause a form of Polycystic Kidney Disease. The molecular mechanism underlying Dnajb11-related kidney disease involves an impairment in the processing of Polycystin-1, which is the protein most often mutated in Autosomal Dominant Polycystic Kidney Disease (ADPKD). Whether additional co-factors are necessary for DNAJB11-dependent PC-1 processing has so far remained unclear. In this study, we perform an unbiased interaction proteomics screen for DNAJB11 interacting proteins. We identify two highly homologous proteins, SDF2 and SDF2L1, as strong interaction partners of DNAJB11. Using newly established knockout cell lines we demonstrate a profound interdependence of DNAJB11 and SDF2/SDF2L1. Furthermore, we show that concomitant loss of SDF2 and SDF2L1 impairs PC-1 processing and that SDF2 or SDF2L1 are elementary subunits of the DNAJB11 complex required for normal PC-1 processing. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-10-15 |
| AnnouncementXML | Submission_2025-10-15_00:23:29.576.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Stefan Tholen |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-02-10 04:59:44 | ID requested | |
| ⏵ 1 | 2025-10-15 00:23:30 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: DNAJB11, DatasetType:Proteomics |
Contact List
| Prof. Dr. Michael Koettgen | |
|---|---|
| contact affiliation | University Medical Center Freiburg |
| contact email | michael.koettgen@uniklinik-freiburg.de |
| lab head | |
| Stefan Tholen | |
| contact affiliation | Uniklinik Freiburg |
| contact email | stefan.tholen@uniklinik-freiburg.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097087/ |
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