PXD060604-1

PXD060604 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Plasma-derived Extracellular Vesicles reveals different biomarkers for monitoring visceral leishmaniasis progression
Description	Visceral leishmaniasis (VL), the most severe form of leishmaniasis, lacks standardized and validated early predictors for cure and relapse, which is especially important for individuals at high-risk. Here, we searched for protein biomarkers in plasma extracellular vesicles (EVs). EVs from patients with active VL (n=12) and patients at post-treatment intervals (1, 3, and 6 months; n=12 per time interval) were analysed by tandem mass spectrometry coupled to liquid chromatography (LC-MS/MS) to characterize their proteomic profiles. Six candidate biomarkers were further tested by ELISA using total plasma. The comparison of the protein profiles of plasma EVs identified 132 human proteins differentially abundant in active VL compared to successfully cured patients, with 64 upregulated and 68 downregulated proteins in VL. Additionally, the analysis highlighted pathogenic mechanisms associated with VL and pathways associated to effective cure. Levels of SAA, and ITGB1 proteins showed a differential expression when analyzed in total plasma, emerging as promising easy-to-measure biomarkers for patients management. Remarkably, proteins from Leishmania spp. were identified in the EV samples, highlighting a novel source of parasite-derived biomarkers in human samples. Plasma EVs contain protein biomarkers to monitor VL treatment success, with some of them detectable using total plasma at 1 month after treatment. This study also provides a proteomic landscape of plasma derived EVs involved in VL, offering insights into disease pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-08-27
AnnouncementXML	Submission_2025-08-27_07:17:54.013.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Montero Calle
SpeciesList	scientific name: Leishmania infantum; NCBI TaxID: 5671; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-09 11:50:09	ID requested
⏵ 1	2025-08-27 07:17:54	announced

10.3389/FIMMU.2025.1646335;

submitter keyword: proteomics, visceral leishmaniasis,extracellular vesicles, patients., cure, plasma, biomarkers

Rodrigo Barderas
contact affiliation	Chronic Disease Program (UFIEC), Instituto de Salud Carlos III, Majadahonda, Spain.
contact email	r.barderasm@isciii.es
lab head
Ana Montero Calle
contact affiliation	Instituto de Salud Carlos III
contact email	ana.monteroc@isciii.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060604
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Plasma-derived Extracellular Vesicles reveals different biomarkers for monitoring visceral leishmaniasis progression