PXD060590-1

PXD060590 is an original dataset announced via ProteomeXchange.

Title	Tyrosine/Lysine-Targeting Urazole Cross-Linkers for Protein Structural Analysis
Description	Chemical cross-linking mass spectrometry (XL-MS) is essential for studying protein structures and interactions. While lysine-targeted cross-linkers dominate current applications, this study introduces two urazole-derived MS-cleavable cross-linkers for tyrosine and lysine residues. A homobifunctional cross-linker (SBT) targets tyrosine via electrochemical click chemistry, while a heterobifunctional cross-linker (SCT) binds both lysine and tyrosine. Their efficiency was validated through reactions with peptides, proteins, and complexes. Additionally, AlphaFold 3-predicted BSA dimer structures were compared with crystallographic data, revealing improved structural coverage. These cross-linkers expand the XL-MS toolkit, enhancing protein structural analysis accuracy and scope.
HostingRepository	iProX
AnnounceDate	2025-02-07
AnnouncementXML	Submission_2025-02-07_19:03:54.665.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhonglin Wei
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-07 19:03:37	ID requested
⏵ 1	2025-02-07 19:03:55	announced

Dataset with its publication pending

submitter keyword: Cross-linking mass spectrometry, Electrochemical click reaction, Tyrosine residue reactive

Zhonglin Wei
contact affiliation	Jilin University
contact email	zlwei@jlu.edu.cn
lab head
Zhonglin Wei
contact affiliation	Jilin University
contact email	zlwei@jlu.edu.cn
dataset submitter

iProX dataset URI