PXD060582 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cryo-EM structure of the CDK11-cyclin L-SAP30BP complex reveals mechanisms of CDK11 regulation |
| Description | The cyclin-dependent kinase CDK11 functions in transcription, mitotic progression, and mRNA splicing. Specifically, spliceosome activation during the B to Bact transition depends on phosphorylation of the U2 snRNP component SF3B1 by the CDK11-cyclin L-SAP30BP complex. Here, we present the structure of this spliceosome-activating CDK-cyclin complex, determined by cryogenic electron microscopy at 2.3 Å resolution. Our structure and biochemical experiments show that SAP30BP forms extensive interactions with cyclin L2, thereby stabilising it, and forms critical interactions with the C-terminal kinase lobe of CDK11. Destabilisation of cyclin L2 in the absence of SAP30BP suggests that these principles are applicable to all CDK11-cyclin L complexes. The structure of the CDK11-cyclin L2-SAP30BP complex bound to the inhibitor OTS964, a clinical, non-covalent, high-affinity CDK11 inhibitor and a comparison to OTS964-bound off-target complexes provide insight into the mechanism of OTS964 selectivity and specificity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-24 |
| AnnouncementXML | Submission_2026-03-24_07:09:19.867.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Graeme Benstead-Hume |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-02-07 04:10:27 | ID requested | |
| ⏵ 1 | 2026-03-24 07:09:20 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: CDK11-cyclin, L-SAP30BP |
Contact List
| Jyoti Choudhary |
|---|
| contact affiliation | ICR, Functional Proteomics |
| contact email | jyoti.choudhary@icr.ac.uk |
| lab head | |
| Graeme Benstead-Hume |
|---|
| contact affiliation | ICR |
| contact email | ghume@icr.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060582
- Label: PRIDE project
- Name: Cryo-EM structure of the CDK11-cyclin L-SAP30BP complex reveals mechanisms of CDK11 regulation
