PXD060554-1

PXD060554 is an original dataset announced via ProteomeXchange.

Title	A reference dataset of O-GlcNAc proteins in quadriceps skeletal muscle from mice
Description	A key nutrient sensing process in all animal tissues is the dynamic attachment of O-linked N-acetylglucosamine (O-GlcNAc). Determining the targets and roles of O-GlcNAc glycoproteins has the potential to reveal insights into healthy and diseased metabolic states. In cell studies, thousands of proteins are known to be O-GlcNAcylated, but reference datasets for most tissue types in animals are lacking. Here, we apply a chemoenzymatic labeling study to compile a high coverage dataset of quadriceps skeletal muscle O-GlcNAc glycoproteins from mice. Our dataset contains over 550 proteins, and >80% of the dataset matched known O-GlcNAc proteins. This dataset was further annotated via bioinformatics, revealing the distribution, protein interactions, and gene ontology (GO) functions of these skeletal muscle proteins. We compared these quadriceps glycoproteins with a high-coverage O-GlcNAc enrichment profile from mouse hearts and describe the key overlap and differences between these tissue types. Quadriceps muscles can be used for biopsies, so we envision this dataset to have potential biomedical relevance in detecting aberrant glycoproteins in metabolic diseases and physiological studies. This new knowledge adds to the growing collection of tissues with high-coverage O-GlcNAc profiles, which we anticipate will further the systems biology of O-GlcNAc mechanisms, functions, and roles in disease.
HostingRepository	PRIDE
AnnounceDate	2026-07-21
AnnouncementXML	Submission_2026-07-21_15:11:50.919.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhengping Yi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-06 10:36:17	ID requested
⏵ 1	2026-07-21 15:11:51	announced

10.1093/glycob/cwaf005;

Jaiswal R, Liu Y, Petriello M, Zhang X, Yi Z, Fehl C, A reference dataset of O-GlcNAc proteins in quadriceps skeletal muscle from mice. Glycobiology, 35(3):(2025) [pubmed]

ProteomeXchange project tag: Skeletal Muscle Proteome (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Glycoproteomics (B/D-HPP)

submitter keyword: glycoproteomics, skeletal muscle,O-GlcNAc, mouse quadriceps, tissue atlas

Zhengping Yi
contact affiliation	Pharmaceutical Science, EACPHS,Wayne State University,Detroit, MI, USA
contact email	zhengping.yi@wayne.edu
lab head
Zhengping Yi
contact affiliation	Wayne State University
contact email	zhengping.yi@wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060554
     1. Label: PRIDE project
     2. Name: A reference dataset of O-GlcNAc proteins in quadriceps skeletal muscle from mice