PXD060538-1

PXD060538 is an original dataset announced via ProteomeXchange.

Title	A Water-Soluble Aggregation-Induced Emission Photosensitizer with Intrinsic Antibacterial Activity as an Antiplanktonic and Antibiofilm Therapeutic Agent
Description	Photosensitizers (PS)s with aggregation-induced emission (AIE) properties have gained popularity in treating bacterial infections. However, most AIE PSs have poor water solubility and low selectivity, which greatly limit their applications in biological systems. Herein, we report a water-soluble and bacteria-targeting AIE PS, TPA-1, which showed minimum cytotoxicity towards human cells with and without light irradiation. Without light irradiation, TPA-1 acts as a narrow-spectrum antibacterial agent, eradicating planktonic S. aureus and inhibiting their biofilm formation. The mechanism study showed that TPA-1 targets S. aureus membrane, inhibits the supercoiling activity of S. aureus DNA gyrase, and causes downregulation of mulitple essential proteins. With light irradiation, TPA-1 showed excellent antiplanktonic and antibiofilm activities against S. aureus and P. aeruginosa by generating reactive oxygen species that cause membrane damage. Furthermore, TPA-1 under light irradiation can significantly reduce the number of viable bacteria in biofilms and promote wound healing in methicillin-resistant S. aureus (MRSA) and P. aeruginosa-infected mice models
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_07:00:00.154.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wai Po Kong
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-05 21:41:43	ID requested
⏵ 1	2025-05-07 07:00:01	announced

10.1021/acs.jmedchem.5c00403;

Hung CH, Chan KH, Kong WP, Du RL, Ding K, Liang Z, Wang Y, Wong KY, A Water-Soluble Aggregation-Induced Emission Photosensitizer with Intrinsic Antibacterial Activity as an Antiplanktonic and Antibiofilm Therapeutic Agent. J Med Chem, 68(8):8768-8785(2025) [pubmed]

submitter keyword: LC-MS, biofilm,Staphylococcus aureus

Kwok-yin Wong
contact affiliation	Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University
contact email	kwok-yin.wong@polyu.edu.hk
lab head
Wai Po Kong
contact affiliation	Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University
contact email	wai-po-ball.kong@connect.polyu.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060538

PRIDE project URI

• PRIDE
  1. PXD060538
     1. Label: PRIDE project
     2. Name: A Water-Soluble Aggregation-Induced Emission Photosensitizer with Intrinsic Antibacterial Activity as an Antiplanktonic and Antibiofilm Therapeutic Agent