PXD060532-1

PXD060532 is an original dataset announced via ProteomeXchange.

Title	TAZ (WWTR1) Interactome Analysis using a GBP nanotrap based affinity purification approach
Description	The transcriptional co-activator TAZ (WWTR1) plays a crucial role in regulating gene expression related to cell proliferation, differentiation, and tissue homeostasis, particularly within the Hippo signaling pathway. Since TAZ does not directly bind to DNA, it largely relies on its protein-protein interaction network to perform its functions, necessitating a detailed characterization of the TAZ interactome. To achieve this, a GFP-Nanotrap-based affinity purification approach combined with LC-MS/MS protein identification was employed to document a comprehensive list of both known and novel components of the TAZ interactome in myogenic cells.
HostingRepository	PRIDE
AnnounceDate	2025-02-11
AnnouncementXML	Submission_2025-02-11_09:14:37.071.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John McDermott
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-05 15:45:04	ID requested
⏵ 1	2025-02-11 09:14:38	announced

Kelebeev J, MacKeracher A, Miyake T, McDermott JC, TAZ interactome analysis using nanotrap-based affinity purification-mass spectrometry. J Cell Sci, 138(4):(2025) [pubmed]

10.1242/jcs.263527;

submitter keyword: mass spectrometry, affinity-purification, skeletal muscle,TAZ, nanobody

John Mcdermott
contact affiliation	York University
contact email	jmcderm@yorku.ca
lab head
John McDermott
contact affiliation	York University
contact email	jmcderm@yorku.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD060532

PRIDE project URI

• PRIDE
  1. PXD060532
     1. Label: PRIDE project
     2. Name: TAZ (WWTR1) Interactome Analysis using a GBP nanotrap based affinity purification approach