PXD060529-1

PXD060529 is an original dataset announced via ProteomeXchange.

Title	Collagenase-Modified Polydopamine Nanoparticles
Description	Anomalous posterior vitreous detachment (aPVD) is involved in the pathogenesis of many vitreoretinal disorders. This condition is currently managed by vitrectomy, a routine but invasive surgery which is especially challenging in patients with firm vitreoretinal adhesions. Since 1998, pharmacological vitreolysis emerged as a potential replacement or adjunct therapy to vitrectomy. Over the years, most attention was focused on enzymes but their use has been limited mainly due to retinal toxicity. To revive the potential of enzymatic vitreolysis we aimed to immobilize collagenase on the surface of polydopamine nanoparticles to prevent penetration into the retinal layers. We synthesized stable and functional collagenase-modified nanoparticles and were able to induce vitreous liquefaction and complete PVD ex vivo. Moreover, we demonstrated a substantial reduction in retinal toxicity upon injection in bovine vitreoretinal explants. Subsequent in vivo analysis revealed that retinal morphology and function were preserved, in contrast to free collagenase. Despite the presence of vitreous hemorrhages, which can possibly be avoided by optimization of the experimental set-up, we believe to have given the first step in the right direction towards nanotechnology-based enzymatic vitreolysis and to have opened doors for future research
HostingRepository	PanoramaPublic
AnnounceDate	2026-02-10
AnnouncementXML	Submission_2026-02-10_19:49:20.769.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bart Van Puyvelde
SpeciesList	scientific name: Hathewaya histolytica; NCBI TaxID: 1498;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-05 15:05:25	ID requested
⏵ 1	2026-02-10 19:49:21	announced

Minnaert AK, Nguyen VP, Harizaj A, Goemaere I, Van Puyvelde B, Dhaenens M, Sauvage F, Deforce D, Salmaso S, Caliceti P, Braeckmans K, Peynshaert K, Paulus YM, De Smedt SC, Remaut K, Collagenase-modified polydopamine nanoparticles for safe and effective vitreolysis. J Control Release, 383():113753(2025) [pubmed]

submitter keyword: Collagenase, nanoparticles, vitreolysis

Katrien Remaut
contact affiliation	Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy
contact email	Katrien.Remaut@UGent.be
lab head
Bart Van Puyvelde
contact affiliation	ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University
contact email	bart.vanpuyvelde@ugent.be
dataset submitter

Panorama Public dataset URI