PXD060457-1

PXD060457 is an original dataset announced via ProteomeXchange.

Title	TBC1D22B Regulates ER-to-Golgi Trafficking via RAB1B Inactivation and Promotes Oncogenic Programs in Breast Cancer
Description	The anterograde secretory pathway shapes the extracellular environment and the ability of cells to communicate, through the release of cytokines, growth factors, enzymes and extracellular matrix components, and through the regulation of the composition of the plasma membrane (PM). The underlying molecular machinery operates in a concerted and highly regulated manner and is frequently altered in cancer. The Golgi RabGAP protein TBC1D22B is overexpressed in breast cancer and predicts prognosis. Herein, deconvolution of the proximity-labeling and physical interactomes of TBC1D22B, reveals its role in the regulation of anterograde trafficking with liaisons with networks of proteins involved in cell adhesion, and in carbohydrate and lipid metabolism. The function of TBC1D22B in the anterograde pathway was formally demonstrated using the Retention Using Selective Hooks system which synchronizes transport of cargoes from the ER to the Golgi and the PM. TBC1D22B inhibits ER to Golgi transport in a GAP-dependent manner. RAB1B, a key RAB in the early secretory pathway, was found to be a substrate of the TBC1D22B GAP activity, and its silencing phenocopied TBC1D22B overexpression. Thus, TBC1D22B regulates ER to Golgi trafficking through its ability to work as a GAP for RAB1B.
HostingRepository	PRIDE
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_06:18:35.499.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	alessandro cuomo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-04 05:33:08	ID requested
⏵ 1	2025-08-21 06:18:35	announced

Dataset with its publication pending

submitter keyword: TBC1D22B, RAB1B,APEX

Letizia Lanzetti
contact affiliation	- Department of Oncology, University of Torino Medical School, Torino, Italy. - Candiolo Cancer Institute, FPO - IRCCS, Candiolo, Torino, Italy.
contact email	letizia.lanzetti@ircc.it
lab head
alessandro cuomo
contact affiliation	IEO
contact email	alessandro.cuomo@ieo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD060457

PRIDE project URI

• PRIDE
  1. PXD060457
     1. Label: PRIDE project
     2. Name: TBC1D22B Regulates ER-to-Golgi Trafficking via RAB1B Inactivation and Promotes Oncogenic Programs in Breast Cancer