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PXD060454-1

PXD060454 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArgonaute Alg-1 IP Caenorhabditis elegans
DescriptionThe Argonautes (AGOs) are widely expressed, evolutionarily conserved RNA binding proteins that play an important role in gene expression regulation. The AGOs bind to small regulatory noncoding RNAs such as micro RNAs (miRNAs), short interfering RNAs (siRNAs), Piwi-interacting RNAs (piRNAs) etc. The small regulatory noncoding RNAs serve the function of guiding the AGOs to the right target RNAs by complementary base pairing. Additionally, the AGOs interact with GW182 (TNRC6A/-B/-C) proteins and together with small RNAs, they form an effector ribonucleo protein complex named, RNA Induced Silencing Complex (RISC) that regulates several aspects of transcriptional and post-transcriptional gene expression. ALG-1 (Argonaute Like Gene) and ALG-2 are the AGO proteins in C. elegans that are required for miRNA mediated gene expression regulation. Our efforts towards the characterization of the protein complexes comprised of ALG-1 led to the identification of DPF-3, a conserved protease belonging to clinically relevant Di Peptidyl Peptidase IV family, as the novel interacting partner of ALG-1. We have further explored the role of DPF-3 in AGO regulation.
HostingRepositoryPRIDE
AnnounceDate2025-03-06
AnnouncementXMLSubmission_2025-03-05_23:23:02.358.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJan Seebacher
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-04 04:44:16ID requested
12025-03-05 23:23:02announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: pulldown,C. elegans, protein interactions, LC-MS, IP-MS, MaxQuant, argonautes, einprot, Orbitrap Fusion
Contact List
Rajani Gudipati
contact affiliationCentre for Advanced Technology/ Adam Mickiewicz University, PoznaƄ, Poland
contact emailrajani.gudipatti@amu.edu.pl
lab head
Jan Seebacher
contact affiliationFMI Basel
contact emailjan.seebacher@fmi.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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