PXD060441-1

PXD060441 is an original dataset announced via ProteomeXchange.

Title	Identification of F-Box Proteins in ABA-regulated Seed Germination: Interaction of GASA1 signaling peptide and ABA-Induced Ubiquitination in Arabidopsis
Description	Large gene families and the frequent overlapping functions of homologous genes remain a major challenge for functional genetics in plants. In recent years, the development of homology-based gene silencing using computationally generated artificial microRNAs (amiRNAs) has been demonstrated to be a promising tool for functional genomics in plants. In this study, through a forward genetics screen, we isolated an abscisic acid-insensitive amiRNA line targeting five previously uncharacterized F-box Insensitive to ABA (FIA) genes. Notably, a triple mutant in the identified FIA genes FIA1, FIA3 and FIA4 (f1/f3/f4), that are expressed in germinating seeds, exhibited ABA insensitivity in the inhibition of seed germination. In contrast, this ABA insensitivity was not observed in a double mutant of two FIA genes FIA1 and FIA4 (f1/f4). Further investigation of the FIA1 interactome using F box decoy lines revealed the gibberellin (GA)-responsive GASA1 protein that has been reported to encode a small stress signaling peptide as an interacting partner. We found that abscisic acid promoted the ubiquitination of GASA1 in Arabidopsis, leading to its degradation via the 26S proteasome pathway. Together, our study reveals that abscisic acid represses seed germination through FIA proteins and regulates the FIA interactor, GA-responsive GASA1.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:21:22.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Majid Ghassemian
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-03 13:00:05	ID requested
⏵ 1	2026-06-07 16:21:23	announced

Kasera M, Ceciliato PHO, Lopez BNK, Hauser F, Gendron JM, Schroeder JI, Identification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination. Philos Trans R Soc Lond B Biol Sci, 380(1927):20240233(2025) [pubmed]

10.1098/rstb.2024.0233;

submitter keyword: signalling,arabidopsis, ABA

Julian Schroeder
contact affiliation	Cell and Developmental Biology Department, School of Biological Sciences, University of California, San Diego, La Jolla, CA, USA
contact email	jischroeder@ucsd.edu
lab head
Majid Ghassemian
contact affiliation	Scientist
contact email	mghassem@ucsd.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060441
     1. Label: PRIDE project
     2. Name: Identification of F-Box Proteins in ABA-regulated Seed Germination: Interaction of GASA1 signaling peptide and ABA-Induced Ubiquitination in Arabidopsis