PXD060435-1

PXD060435 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Enhanced STEAP4 ubiquitination in Obesity: Insights from Integrated Proteome and Ubiquitylome Analysis of Visceral Adipose Tissue
Description	We obtained the global proteomic and ubiquitylomic data of epididymal VAT from lean and obese male mice by mass spectrometry. Our proteomic analyses revealed significant changes of metabolic pathways involved in fatty acid, acyl-CoA and branched chain amino acids metabolism in obese VAT. Intriguingly, our integrated analysis of proteomic and ubiquitylomic data highlighted a discordance in the quantity changes of certain proteins and their ubiquitination levels. Notably, STEAP4 exhibited a markedly reduced protein level coupled with an enhanced K48-linked ubiquitination, suggesting a potential role for ubiquitination-mediated proteasome degradation in VAT dysfunction. Collectively, this study introduces the first combined proteomic and ubiquitylomic examination of murine VAT, offering novel insights and potential therapeutic targets for obesity.
HostingRepository	PRIDE
AnnounceDate	2026-01-23
AnnouncementXML	Submission_2026-01-23_03:12:25.990.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhao Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	ubiquitinylated lysine
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-03 11:01:17	ID requested
⏵ 1	2026-01-23 03:12:26	announced

Publication List

10.1016/j.mcpro.2025.101474;
Li Y, Li J, Wang G, Yang S, Liu D, Wen H, Zhang M, Duan C, Yu M, Wang S, Guo S, Chen X, Wang L, Enhanced STEAP4 Ubiquitination in Obesity: Insights From Combined Proteome and Ubiquitylome Analysis of Visceral Adipose Tissue. Mol Cell Proteomics, 25(1):101474(2025) [pubmed]

10.1016/j.mcpro.2025.101474;

Li Y, Li J, Wang G, Yang S, Liu D, Wen H, Zhang M, Duan C, Yu M, Wang S, Guo S, Chen X, Wang L, Enhanced STEAP4 Ubiquitination in Obesity: Insights From Combined Proteome and Ubiquitylome Analysis of Visceral Adipose Tissue. Mol Cell Proteomics, 25(1):101474(2025) [pubmed]

Keyword List

submitter keyword: ubiquitylome, obesity, STEAP4,proteome

submitter keyword: ubiquitylome, obesity, STEAP4,proteome

Contact List

Yuhao Li
contact affiliation	Institute of Immunology PLA, Army Medical University (Third Military Medical University), Chongqing 400038, China
contact email	liyuhao616@163.com
lab head
Yuhao Li
contact affiliation	Institute of Immunology PLA, Army Medical University (Third Military Medical University), Chongqing 400038, China
contact email	liyuhao616@163.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD060435
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD060435

PRIDE project URI

Repository Record List

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