PXD060429-1

PXD060429 is an original dataset announced via ProteomeXchange.

Title	Cross-organelle Coordination to Advance Plant Cytochrome P450 Functionality in Yeast
Description	Sustainable production of plant secondary metabolites remains a significant challenge. Engineered yeast platforms offer a promising alternative, yet reconstituting plant cytochrome P450s (CYPs), key enzymes in these pathways, remains a major burden. Plant Membrane-Steroid-Binding Proteins (MSBPs) have been suggested to enhance CYP functionality, possibly by facilitating CYP-CYP reductase (CPR) interactions. In this study, Arabidopsis thaliana MSBP1 (AtMSBP1) was engineered with diverse plant CYPs to assess its broad CYP activity-enhancing effects. Surprisingly, CYP-CPR interactions are not essential for AtMSBP1’s function. Instead, AtMSBP1 orchestrates inter-organelle communication, involving not only the endoplasmic reticulum (ER), but mitochondria and vacuoles. Co-expression of AtMSBP1 with CYPs triggered significant subcellular reorganization, including tubular ER expansion, increased mitochondrial volume, and vacuole fission. This study highlights the critical role of organelle coordination in supporting CYP functionality, emphasizing a shift from traditional ER-based approaches to engineering cross-organelle interactions and tailoring the cellular microenvironment to enhance plant CYP activity in yeast.
HostingRepository	PRIDE
AnnounceDate	2025-09-22
AnnouncementXML	Submission_2025-09-22_15:17:06.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Songbo Wei
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-03 10:49:42	ID requested
⏵ 1	2025-09-22 15:17:06	announced

Dataset with its publication pending

submitter keyword: LC-MSMS,Yeast

Yanran Li
contact affiliation	UC San Diego
contact email	yal152@ucsd.edu
lab head
Songbo Wei
contact affiliation	University of California, Riverside
contact email	songbow@ucr.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060429
     1. Label: PRIDE project
     2. Name: Cross-organelle Coordination to Advance Plant Cytochrome P450 Functionality in Yeast