PXD060424-1

PXD060424 is an original dataset announced via ProteomeXchange.

Title	Proteic characterization of the IgM triplet involved in the diagnosis of congenital toxoplasmosis
Description	Congenital toxoplasmosis is a fetal infection following the transplacental transmission of Toxoplasma gondii in a mother who seroconverted during pregnancy. Neonatal diagnosis has recently been improved through the identification by L’Ollivier et al. and Peyclit et al. of a congenital toxoplasmosis pathognomonic marker: the IgM triplet, corresponding to three bands of high molecular weight of 75, 90 and 100 kDa respectively found on the immunoblot pair profile mother-child. This is a new concept, as these three IgM bands do reflect an immunological response against proteins involved in mother-to-child transmission of Toxoplasma gondii. These proteins could be Toxoplasma gondii secreted effectors or not playing a role in the processes of invasion, modulation of the host cell immune system as well as in parasitic virulence. In this study, immunoproteomic techniques allowed us to identify 32 interesting protein spots on immunoblot, including 4 interesting spots specific to the IgM triplet. After protein identification of these spots by LC-MS/MS technique, we showed here that several Toxoplasma gondii proteins were good candidates for the IgM triplet. It turns out that each of these proteins is, directly or indirectly, involved in the process of cellular invasion and therefore probably in the process of transplacental invasion of Toxoplasma gondii. The identification of these proteins opens several fields of future diagnostic and therapeutic research that would improve management of congenital toxoplasmosis.
HostingRepository	PRIDE
AnnounceDate	2025-12-08
AnnouncementXML	Submission_2025-12-07_17:29:13.622.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060424
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hélène Diemer
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: NEWT:5811;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-03 07:16:35	ID requested
⏵ 1	2025-12-07 17:29:14	announced

10.1051/parasite/2025065;

Carreno M, Villard O, Villena I, Peyclit L, Diemer H, Schaeffer-Reiss C, L'Ollivier C, Protein characterization of the IgM triplet involved in the diagnosis of congenital toxoplasmosis. Parasite, 32():72(2025) [pubmed]

10.6019/PXD060424;

submitter keyword: 2D gel,Toxoplasma gondii, immunoproteomics, IgM triplet, LC-MS/MS

Sarah Cianferani
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg, CNRS, IPHC UMR 7178, 67087 Strasbourg, France
contact email	sarah.cianferani@unistra.fr
lab head
Hélène Diemer
contact affiliation	LSMBO
contact email	hdiemer@unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060424
     1. Label: PRIDE project
     2. Name: Proteic characterization of the IgM triplet involved in the diagnosis of congenital toxoplasmosis