PXD060420-1

PXD060420 is an original dataset announced via ProteomeXchange.

Title	Gut Microbially Produced Bile Acid Metabolite Lengthens Circadian Period in Host Intestinal Cells
Description	Although it has been shown that host health, circadian signaling, and the microbiome are tightly connected, the mechanistic links remain unclear. We identified a bile acid metabolite, lithocholic acid (LCA), as a circadian modulator by screening a focused library of gut microbial metabolites. Out of the larger class of compounds, LCA, and to a lesser extent isoLCA, uniquely lengthened the circadian period of hPer2 transcription in a dose-responsive manner in human colonic cells. LCA modulated the casein kinase 1 / (CK1/)-protein phosphatase 1 (PP1) feedback loop and stabilized core clock protein cryptochrome 2 (CRY2). Furthermore, we showed that LCA feeding alters circadian transcription in mouse colons. Thus, our work identifies LCA as an endogenous molecular link between host circadian biology and the microbiome, providing a new avenue to examine host health.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_19:54:59.630.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD060420
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Robyn Eisert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	L-methionine sulfoxide; phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-03 04:55:37	ID requested
⏵ 1	2026-05-24 19:55:00	announced

Powell CE, McSween AM, Dohnalov, á L, Kim CH, Eisert RJ, Sun ZJ, Seo HS, Marquardt V, Dhe-Paganon S, Thaiss CA, Devlin AS, Gut microbiome-produced bile acid metabolite lengthens the circadian period in host intestinal cells. Proc Natl Acad Sci U S A, 123(11):e2506313123(2026) [pubmed]

10.6019/PXD060420;

10.1073/pnas.2506313123;

submitter keyword: microbiome,circadian rhythm, Lithocholic acid, HT-29 cells, phosphoproteomics

A. Sloan Devlin
contact affiliation	Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School; Boston, MA, United States
contact email	sloan_devlin@g.harvard.edu
lab head
Robyn Eisert
contact affiliation	Harvard Medical School
contact email	robyn_eisert@hms.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060420
     1. Label: PRIDE project
     2. Name: Gut Microbially Produced Bile Acid Metabolite Lengthens Circadian Period in Host Intestinal Cells