PXD060418-1

PXD060418 is an original dataset announced via ProteomeXchange.

Title	TRPV6 essentially contributes to Ca2+ influx in endometrial epithelial cells and female mouse fecundity
Description	The Ca2+-selective transient receptor potential vanilloid 6 (TRPV6) channel plays a fundamental role in the mouse female and male reproductive system. In mouse endometrium the TRPV6 protein is highly expressed in epithelial cells (MEECs). Using Ca2+ imaging and patch clamp analysis we found that TRPV6 contributes to the cytosolic Ca2+ level, more specifically to the Ca2+ influx into the MEECs. As compared to wild-type cells, Trpv6-deficient MEECs reveal a significantly reduced frequency of spontaneous cytosolic Ca2+ oscillations, shown in both isolated cells and the complete uterus in situ. Noteworthy, in MEECs we here show for the first time distinct endogenous TRPV6-dependent whole-cell currents detected from primary cells. Our results disclose a previously unknown physiological role for TRPV6 in the regulation of endometrial Ca2+ homeostasis and its impact on female mouse fecundity, representing a basis for further investigation of reproductive impairments e.g. associated with defective Ca2+ regulation in women.
HostingRepository	PRIDE
AnnounceDate	2025-10-13
AnnouncementXML	Submission_2025-10-12_18:21:30.486.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060418
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Claudia Fecher-Trost
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-03 03:56:48	ID requested
⏵ 1	2025-10-12 18:21:31	announced

10.6019/PXD060418;

10.1007/s00018-025-05857-9;

Sota A, Beck A, Wartenberg P, Gehl AL, Winter M, Wissenbach U, Freichel M, Meyer MR, Boehm U, Flockerzi V, Fecher-Trost C, Weissgerber P, signaling and female mouse fecundity. Cell Mol Life Sci, 82(1):346(2025) [pubmed]

submitter keyword: MEEC, fecundity, endometrium,TRPV6, MESC

Petra Weissgerber
contact affiliation	Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University, 66421 Homburg, Germany
contact email	petra.weissgerber@uni-saarland.de
lab head
Claudia Fecher-Trost
contact affiliation	PZMS
contact email	claudia.fecher-trost@uks.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD060418

PRIDE project URI

• PRIDE
  1. PXD060418
     1. Label: PRIDE project
     2. Name: TRPV6 essentially contributes to Ca2+ influx in endometrial epithelial cells and female mouse fecundity