⮝ Full datasets listing
PXD060411-1
PXD060411 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A functional map of phosphoprotein phosphatase regulation identifies an evolutionary conserved reductase for the active site |
| Description | Ser/Thr phosphoprotein phosphatases (PPPs, PP1-PP7) are conserved metalloenzymes and central regulators of cellular signaling in eukaryotes, but the details of their assembly and regulation are not fully understood. To address this, we performed genome-wide CRISPR knockout and focused high resolution base editor screens to functionally interrogate PPP regulation in human cells. This uncovered several novel regulators including the poorly studied reductase CYB5R4 which we identify as an evolutionarily conserved activator of PP4 and PP6. CYB5R4 heme binding is essential for this function and mechanistically involves the reduction of the metal ions in the active site. Importantly, this function of CYB5R4 controls the cellular response to DNA damage inducing chemotherapeutic agents. In sum we provide a high resolution functional map of PPP regulation and uncover that active site metal ion redox state is an integral regulatory mechanism of PPP biology. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-22 |
| AnnouncementXML | Submission_2026-04-22_02:57:30.511.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Arminja Nadine Kettenbach |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-02-02 09:45:29 | ID requested | |
| ⏵ 1 | 2026-04-22 02:57:31 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Phosphatase, biogenesis, PP2A-like, DatasetType:Proteomics |
Contact List
| Arminja Kettenbach | |
|---|---|
| contact affiliation | The Geisel School of Medicine at Dartmouth |
| contact email | arminja.n.kettenbach@dartmouth.edu |
| lab head | |
| Arminja Nadine Kettenbach | |
| contact affiliation | Dartmouth |
| contact email | Arminja.N.Kettenbach@Dartmouth.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097024/ |
