PXD060402-1

PXD060402 is an original dataset announced via ProteomeXchange.

Title	sRNA-mediated crosstalk between cell wall stress and galactose metabolism in Staphylococcus aureus
Description	Staphylococcus aureus is an opportunistic pathogen responsible for a wide range of diseases in humans. During infections, this bacterium is exposed to various stresses that target its cell wall, such as oxidative or acid environments as well as various cell wall-acting antimicrobials. S. aureus has effective regulatory systems for responding to environmental stresses enabling the expression of factors necessary for its survival. Bacterial small RNAs (sRNAs) play a crucial role in this adaptation process. In this study, we show that RsaOI, an S. aureus sRNA, accumulates under acid stress conditions. This response is mediated via the two-component system VraSR, which is associated with the cell wall damage response. As a component of the VraSR regulon, RsaOI contributes to the survival of S. aureus under acid stress and affects its susceptibility to glycopeptide antibiotics. To identify RsaOI-dependent changes in the abundance of individual proteins, we analyzed by quantitative proteomics which protein levels were affected by RsaOI expression. Our findings reveal that RsaOI targets lacABCDFEG operon, which encodes components of tagatose pathway, a unique mechanism responsible for galactose metabolism in S. aureus. By antisense base pairing near the ribosome-binding site of lacD, RsaOI inhibits the expression of this gene, encoding tagatose-6-phosphate aldolase. This regulation disrupts the tagatose pathway, impairing galactose utilization in S. aureus. Furthermore, we demonstrated that disruption of the lac operon repression leads to significant alterations in the cell morphology and cell envelope structure. These findings highlight the role of RsaOI in the mediation between cell wall stress responses and specific metabolic pathway.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-27_16:11:10.235.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julie Hardouin
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-31 09:40:39	ID requested
⏵ 1	2025-07-27 16:11:11	announced

10.1093/nar/gkaf616;

Germain M, Robin H, Le Huyen KB, Massier S, Nalpas N, Hardouin J, Bouloc P, Rouillon A, Chabelskaya S, sRNA-mediated crosstalk between cell wall stress and galactose metabolism in Staphylococcus aureus. Nucleic Acids Res, 53(13):(2025) [pubmed]

submitter keyword: small RNA regulation, Galactose metabolism, Acidic stress,Staphylococcus aureus

Julie Hardouin
contact affiliation	Université de Rouen - Plateforme PISSARO
contact email	julie.hardouin@univ-rouen.fr
lab head
Julie Hardouin
contact affiliation	Université de Rouen - laboratoire PBS
contact email	julie.hardouin@univ-rouen.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD060402

PRIDE project URI

• PRIDE
  1. PXD060402
     1. Label: PRIDE project
     2. Name: sRNA-mediated crosstalk between cell wall stress and galactose metabolism in Staphylococcus aureus