PXD060377-1

PXD060377 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic analysis of dried blood from the Sepsis Characterization in Kilimanjaro (SICK) study
Description	Venous-EDTA whole blood was captured on 20 microliter Mitra devices at days 0,1,3,28 after hospital admission with Sepsis-2 criteria. Mitra tips were processed in Matrix tubes using deoxycholate-assisted in solution trypsin digestion. Approximately 1 mg of digests was enriched for N-glycopeptidesusing spin tips packed with Polyhydroxyethyl A followed by serial enrichment of phosphopeptides from the flow through using Ti-IMAC. LC-MS/MS of unenriched and phosphopeptide-enriched samples used microflow LC (30 samples-per-day) and Evosep LC (60 SPD), respectively, with data-dependent acquisition using an Orbitrap Astral MS. LC-MS/MS of N-glycopeptide-enriched samples used an Evosep LC (60 SPD) and either stepped-collision energy data-dependent acquisition (Exploris 480) or separate DDA and DIA with a 35% NCE using the Orbitrap Astral. Data analysis used Spectronaut and Glyco-Decipher.
HostingRepository	MassIVE
AnnounceDate	2025-02-03
AnnouncementXML	Submission_2025-02-03_17:52:41.404.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matt Foster
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Acetyl; Carbamidomethyl; N-linked glycan core; Phospho
Instrument	Orbitrap Astral; Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-30 11:58:48	ID requested
⏵ 1	2025-02-03 17:52:41	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Mitra device, whole blood, N-glycoproteome, phosphoproteome, microflow, DatasetType:Proteomics

submitter keyword: Mitra device, whole blood, N-glycoproteome, phosphoproteome, microflow, DatasetType:Proteomics

Contact List

Matthew W. Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
lab head
Matt Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v09/MSV000097000/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v09/MSV000097000/