PXD060357 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparative analysis of platelet-derived extracellular vesicles protein extraction methodologies for mass spectrometry |
| Description | This study presents a comparative study of different methodologies for the extraction of proteins from platelet derived-extracellular vesicles (pEV) prior to subsequent mass spectrometry (MS) analysis. Protein isolation is performed through three different methodologies based on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), organic solvent precipitation (OSP) or magnetic beads (MB), followed by protein digestion and sample acquisition by LC-MS/MS. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-08-25 |
| AnnouncementXML | Submission_2025-08-24_17:20:20.428.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD060357 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Carmen Raez Meseguer |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
| 0 | 2025-01-30 02:54:13 | ID requested | |
| ⏵ 1 | 2025-08-24 17:20:21 | announced | |
Publication List
| 10.6019/PXD060357; |
| R, á, ez-Meseguer C, Amengual-Tugores AM, Forteza-Genestra MA, Orvay-Pintos F, Gomila RM, Martorell-Cresp, í G, Calvo J, Gay, à A, Monjo M, Ramis JM, Comparative Analysis of Platelet-Derived Extracellular Vesicle Protein Extraction Methodologies for Mass Spectrometry. J Proteome Res, 24(8):3931-3942(2025) [pubmed] |
| 10.1021/acs.jproteome.5c00089; |
Keyword List
| submitter keyword: proteomics, proteins, extracellular vesicles, platelets., methodologies,Mass spectrometry |
Contact List
| Joana Maria Ramis Morey |
| contact affiliation | Group of Cell Therapy and Tissue Engineering (TERCIT), Research Institute on Health Sciences (IUNICS), University of the Balearic Islands (UIB), Palma, Spain. Health Research Institute of the Balearic Islands (IdISBa), Palma, Spain. Department of Fundamental Biology and Health Sciences, UIB, Palma, Spain. |
| contact email | joana.ramis@uib.es |
| lab head | |
| Carmen Raez Meseguer |
| contact affiliation | Group of Cell Therapy and Tissue Engineering (TERCIT), Research Institute on Health Sciences (IUNICS), University of the Balearic Islands (UIB), Palma, Spain. Health Research Institute of the Balearic Islands (IdISBa), Palma, Spain. Department of Fundamental Biology and Health Sciences, UIB, Palma, Spain. |
| contact email | carmen.raez@uib.es |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060357
- Label: PRIDE project
- Name: Comparative analysis of platelet-derived extracellular vesicles protein extraction methodologies for mass spectrometry