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PXD060357-1

PXD060357 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative analysis of platelet-derived extracellular vesicles protein extraction methodologies for mass spectrometry 
DescriptionThis study presents a comparative study of different methodologies for the extraction of proteins from platelet derived-extracellular vesicles (pEV) prior to subsequent mass spectrometry (MS) analysis. Protein isolation is performed through three different methodologies based on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), organic solvent precipitation (OSP) or magnetic beads (MB), followed by protein digestion and sample acquisition by LC-MS/MS.
HostingRepositoryPRIDE
AnnounceDate2025-08-25
AnnouncementXMLSubmission_2025-08-24_17:20:20.428.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD060357
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCarmen Raez Meseguer
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-01-30 02:54:13ID requested
12025-08-24 17:20:21announced
Publication List
10.6019/PXD060357;
R, á, ez-Meseguer C, Amengual-Tugores AM, Forteza-Genestra MA, Orvay-Pintos F, Gomila RM, Martorell-Cresp, í G, Calvo J, Gay, à A, Monjo M, Ramis JM, Comparative Analysis of Platelet-Derived Extracellular Vesicle Protein Extraction Methodologies for Mass Spectrometry. J Proteome Res, 24(8):3931-3942(2025) [pubmed]
10.1021/acs.jproteome.5c00089;
Keyword List
submitter keyword: proteomics, proteins, extracellular vesicles, platelets., methodologies,Mass spectrometry
Contact List
Joana Maria Ramis Morey
contact affiliationGroup of Cell Therapy and Tissue Engineering (TERCIT), Research Institute on Health Sciences (IUNICS), University of the Balearic Islands (UIB), Palma, Spain. Health Research Institute of the Balearic Islands (IdISBa), Palma, Spain. Department of Fundamental Biology and Health Sciences, UIB, Palma, Spain.
contact emailjoana.ramis@uib.es
lab head
Carmen Raez Meseguer
contact affiliationGroup of Cell Therapy and Tissue Engineering (TERCIT), Research Institute on Health Sciences (IUNICS), University of the Balearic Islands (UIB), Palma, Spain. Health Research Institute of the Balearic Islands (IdISBa), Palma, Spain. Department of Fundamental Biology and Health Sciences, UIB, Palma, Spain.
contact emailcarmen.raez@uib.es
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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