PXD060312 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Regulation of early-stage tomato fruit growth by the splicing factor RS2Z36 |
| Description | Fruit size and shape are determined by cell division and expansion. In tomato, the model plant for fleshy fruit development, both processes are associated with changes in gene expression including transcription and RNA processing. While several transcription factors have been identified as regulators of genes involved in fruit development, the contribution of splicing regulators remains unknown. Profiling of RNA splicing-related genes revealed stage-preferential expression of several factors, including RS2Z36, a serine/arginine-rich splicing factor highly expressed during the early stages of fruit development. Mutations in RS2Z36 lead to smaller, ellipsoid fruits compared to the wild-type, with histological analysis showing elongated pericarp cells in the ovaries along the longitudinal axis. RNA-seq analysis uncovered numerous alternatively spliced genes involved in different biological processes. Complementary proteome analysis identified proteins with altered abundance and peptides corresponding to novel RNA splice variants, underscoring the role of RS2Z36 in shaping both transcriptome and proteome diversity. Immunofluorescence screening of cell wall proteins further demonstrated an increased abundance of LM6-recognized arabinan epitopes of rhamnogalacturonan-I pectins and arabinogalactan protein (AGP) epitopes in rs2z36-1 pericarp cells compared to the wild type. These findings highlight RS2Z36 as a critical regulator of alternative splicing, impacting fruit development through diverse transcriptome and proteome changes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-15 |
| AnnouncementXML | Submission_2026-06-14_16:13:16.908.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Palak Chaturvedi |
| SpeciesList | scientific name: Solanum lycopersicum; NCBI TaxID: NEWT:4081; |
| ModificationList | acetylated residue; monohydroxylated residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-29 02:41:48 | ID requested | |
| ⏵ 1 | 2026-06-14 16:13:17 | announced | |
Publication List
| 10.1007/s11103-026-01730-w; |
| Vraggalas S, Rosenkranz RRE, Keller M, P, é, rez-P, é, rez Y, Bachiri S, Zehl KJ, Bold J, Simm S, Ghatak A, Weckwerth W, Afjehi-Sadat L, Chaturvedi P, Testillano PS, M, ü, ller-McNicoll M, Zarnack K, Fragkostefanakis S, Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato. Plant Mol Biol, 116(4):(2026) [pubmed] |
Keyword List
| submitter keyword: Splicing factors, Tomato,Fruit growth, Proteomics on Spliceforms |
Contact List
| Palak Chaturvedi |
|---|
| contact affiliation | University of Vienna |
| contact email | palak.chaturvedi@univie.ac.at |
| lab head | |
| Palak Chaturvedi |
|---|
| contact affiliation | University of Vienna |
| contact email | palak.chaturvedi@univie.ac.at |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060312
- Label: PRIDE project
- Name: Regulation of early-stage tomato fruit growth by the splicing factor RS2Z36
