PXD060281-1

PXD060281 is an original dataset announced via ProteomeXchange.

Title	Immunoglobulin A Carries Sulfated and O-acetylated N-glycans Primarily at the Tailpiece Site
Description	A site-specific description of a recently discovered HexNAc-sulfated N-glycans of human immunoglobulin A (IgA) was still pending. Here we provide an in-depth N-glycoproteomic analysis of human serum IgA. This in-depth N-glycoproteomic analysis, developed by us, integrates a new strategy for identifying sulfated and other rare N-glycans in IgA. In our study, we implemented two strategies for identifying the new N-glycan compositions: screening for rare glycopeptides using oxonium marker ions and wildcard search to identify glycans holding a rare modification attached to peptides. The dataset provided here contains primarily IgA N-glycopeptides identified from technical quadruplicates of two commercial human serum IgA samples. A comparison of the N-glycosylation profiles of two commercial human serum IgA samples demonstrated that sulfated N-glycans are mainly present in the tailpiece site. Also, complex-type N-glycan compositions with O-acetylated sialic acid were identified in the tailpiece. Surprisingly, N-glycans bearing glucuronic acid were identified in the commercial IgA samples, but from peptides of contaminant glycoproteins.These N-glycans have not been included in previously published IgA micro-heterogeneity analyses. We expect that a broader micro-heterogeneity description of clinically relevant glycoproteins, such as IgA, can expand the screening for biomarkers or treatment options.
HostingRepository	MassIVE
AnnounceDate	2025-06-25
AnnouncementXML	Submission_2025-06-25_05:07:38.274.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Frania Jaqueline Zuniga Banuelos
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	unknown modification
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-28 06:43:57	ID requested
⏵ 1	2025-06-25 05:07:38	announced

no publication

submitter keyword: immunoglobulin A (IgA), mass spectrometry, glycoproteomics, oxonium marker ions, sulfation, O-acetylation, rare N-glycans, N-glycosylation, DatasetType:Other (glycoproteomics)

Frania Jaqueline Zuniga-Banuelos
contact affiliation	Max Planck Institute for Dynamics of Complex Technical Systems
contact email	zuniga@mpi.magdeburg.de
lab head
Udo Reichl
contact affiliation	Max Planck Institute for Dynamics of Complex Technical Systems
contact email	ureichl@mpi-magdeburg.mpg.de
lab head
Erdmann Rapp
contact affiliation	Max Planck Institute for Dynamics of Complex Technical Systems
contact email	rapp@mpi-magdeburg.mpg.de
lab head
Marcus Hoffmann
contact affiliation	Max Planck Institute for Dynamics of Complex Technical Systems
contact email	mhoffmann@mpi-magdeburg.mpg.de
lab head
Greta Lemke
contact affiliation	Max Planck Institute for Dynamics of Complex Technical Systems
contact email	glemke@mpi-magdeburg.mpg.de
lab head
Frania Jaqueline Zuniga Banuelos
contact affiliation	Max Planck Institute
contact email	zuniga@mpi-magdeburg.mpg.de
dataset submitter

MassIVE dataset URI

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