PXD060262-1

PXD060262 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis integrated with transcriptomics of clubroot resistant and susceptible Brassica napus near-isogenic lines in response to Plasmodiophora brassicae infection
Description	Clubroot disease, caused by Plasmodiophora brassicae, poses a significant global threat to Brassica crop production. Understanding the disease resistance mechanism is vital to assist in the continuous development of clubroot-resistant genetic material. In this study, we used clubroot-resistant (CR) and -susceptible (CS) near-isogenic lines (NILs) of B. napus, carrying resistance of turnip (B. rapa var. rapifera), and conducted transcriptomics and proteomics analysis to characterize the molecular basis of host resistance against clubroot. This study identified genes involved in reactive oxygen species (ROS) scavenging [peroxygenase 3 (BnaA04T0204000WE) and peroxidase 2 (BnaA09T0647200WE)], cell wall modifications [caffeoylshikimate esterase (BnaA04T0244300WE) and shikimate-O-hydroxycinnamoyltransferase (BnaC06T0472200WE)], and glucosinolate biosynthesis [2-oxoglutarate-dependent dioxygenase AOP1 (BnaA01T0266700WE)] that were highly abundant in CR lines. Additionally, disease resistance-related genes, including enhanced disease resistance 2-like (BnaA03T0055600WE) and hairpin-induced family protein YLS9 (BnaA08T0237900WE), were also elevated in the CR lines. In contrast, CS NILs exhibited decreased expression of defense-related genes, increased expression of cell wall-degrading enzymes, and enhanced carbohydrate metabolism, facilitating pathogen proliferation. Integrated analysis of data from the two ‘omics’ approaches showed that the genes related to plant invertase/pectin methylesterase inhibitor (BnaC04T0003100WE), Kelch motif (BnaC02T0374800WE), and laccase (BnaA02T0019200WE) potentially play an important role in host resistance. Antioxidant-related genes such as glucose-6-phosphate dehydrogenase (BnaC09T0029500WE), glutathione S-transferases (BnaA03T0281000WE, BnaA03T0280900WE), catalases (Bnascaffold286T0031400WE), and 4-hydroxyphenylpyruvate dioxygenase (BnaA09T0641500WE) were upregulated in the resistant NILs, potentially protecting cell wall components by ROS scavenging. The identified genes and pathways offer valuable targets for use in breeding aimed at enhancing clubroot resistance.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_17:39:59.087.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Uhrig
SpeciesList	scientific name: Brassica napus (Rape); NCBI TaxID: NEWT:3708;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-27 10:09:09	ID requested
⏵ 1	2025-10-05 17:40:00	announced

10.3390/ijms26189157;

Kaur K, Adhikary D, Kav NNV, Scandola S, Uhrig RG, Rahman H, Infection. Int J Mol Sci, 26(18):(2025) [pubmed]

submitter keyword: proteomics,Brassica napus, clubroot

R. Glen Uhrig
contact affiliation	Department of Biological Sciences, University of Alberta
contact email	ruhrig@ualberta.ca
lab head
Richard Uhrig
contact affiliation	University of Alberta
contact email	ruhrig@ualberta.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD060262

PRIDE project URI

• PRIDE
  1. PXD060262
     1. Label: PRIDE project
     2. Name: Proteomics analysis integrated with transcriptomics of clubroot resistant and susceptible Brassica napus near-isogenic lines in response to Plasmodiophora brassicae infection