PXD060225-1

PXD060225 is an original dataset announced via ProteomeXchange.

Title	Hidden players in plant-insect interactions: effects of third-trophic level symbionts on responses of plants to herbivory
Description	Mutualistic symbionts are increasingly recognized as hidden players in plant-insect interactions. Understanding how symbionts affect plant responses to herbivory provides important insight into the fascinating complexity of terrestrial ecological networks and how we could specifically target symbionts to achieve sustainable pest management. Although the role of herbivore-associated symbionts is gaining increased attention in ecology of plant-insect interactions, how symbionts of members of higher trophic levels affect these interactions is poorly understood. The natural enemies of insect herbivores such as parasitic wasps that are used in biological pest control are associated with mutualistic symbionts (polydnaviruses) that they inject into the herbivore along with eggs in an event of parasitisation. We showed previously that polydnavirus-genes are expressed in the salivary glands of parasitized caterpillars suggesting that third-trophic level symbionts may alter the herbivore’s salivary composition and consequently, affect the way the herbivore interacts with the plant. By using a novel multidisciplinary approach that integrates molecular, chemical, behavioral and field ecology, the proposed project aims to manipulate the phenotype of caterpillars to study the effect of symbiotic viruses of third-trophic-level insects at the plant-herbivore interface. We will address this issue at different levels of biological organization, from plant gene expression to plant-mediated ecological consequences. The project will thereby provide novel fundamental insights into the hidden role played by parasitic wasp symbionts in plant responses to herbivory and these insights will generate important knowledge for pest management programs that focus on polydnaviruses as biological control agents.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:58:01.891.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060225
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Functional Proteomics Platform FPP
SpeciesList	scientific name: Cotesia glomerata; NCBI TaxID: 32391; scientific name: Pieris brassicae; NCBI TaxID: 7116; scientific name: Hyposoter ebeninus; NCBI TaxID: 1045610; scientific name: Brassica oleracea (Wild cabbage); NCBI TaxID: 3712;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-27 01:31:39	ID requested
⏵ 1	2025-08-24 16:58:02	announced

10.6019/PXD060225;

10.1111/ele.70183;

Cusumano A, Urbach S, Jouan V, Vogel H, Dicke M, Volkoff AN, Poelman EH, Convergence in Symbiont-Induced Plant-Mediated Responses to Herbivory: Cascading Effects for Foraging Parasitoids. Ecol Lett, 28(8):e70183(2025) [pubmed]

submitter keyword: LC-MSMS, PDVs,Insect parasitoids

Antonino Cusumano
contact affiliation	Department of Agricultural, Food And Forest Sciences (SAAF) University of Palermo Viale delle Scienze, 90128 Palermo, Italy
contact email	Antonino.cusumano@unipa.it
lab head
Functional Proteomics Platform FPP
contact affiliation	FPP CNRS
contact email	contact@fpp.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD060225

PRIDE project URI

• PRIDE
  1. PXD060225
     1. Label: PRIDE project
     2. Name: Hidden players in plant-insect interactions: effects of third-trophic level symbionts on responses of plants to herbivory