PXD060223-1
PXD060223 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A role for the autophagy receptor NBR1 in the degradation of Tau aggregates |
| Description | Neurofibrillary tangles (NFTs), comprising hyperphosphorylated and aggregated Tau protein, are a primary neuropathological feature of Alzheimer's Disease (AD). In patients, the formation and spread of NFTs across the brain correlate with cognitive decline. However, the mechanisms driving Tau aggregation and leading to the subsequent neuronal dysfunction are not fully understood. In this study, we explored proteomic and phosphoproteomic changes resulting from the seed-induced aggregation of endogenous Tau in human neurons derived from induced pluripotent stem (iPS) cells. We discovered previously undescribed phosphorylation sites on NBR1, an autophagy receptor, which were significantly altered by Tau aggregation in vitro. We further show that NBR1 directly interacts with phosphorylated Tau and Tau aggregates in various cell models. This interaction is associated with autophagic Tau degradation in HEK cells, and siRNA-mediated knockdown of NBR1 significantly increases Tau aggregate levels in iPS-derived neurons. Additionally, we find that NBR1 specifically interacts with phospho-Tau in human AD brain, underscoring the relevance of our findings to the human disease. These insights provide a deeper understanding of the molecular interactions between autophagy receptors and Tau pathology in AD and reveal a role for NBR1 as an important receptor for pathological forms of Tau. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-08-15 |
| AnnouncementXML | Submission_2025-08-15_09:25:36.982.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Marie Neu |
| SpeciesList | scientific name: human; |
| ModificationList | Phospho |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-01-27 00:38:43 | ID requested | |
| ⏵ 1 | 2025-08-15 09:25:37 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Alzheimer's Disease, Tau aggregation, phospho-proteomics, proteomics, autophagy receptor, Tau, iPS-derived neurons, mass spectrometry, DatasetType:Proteomics |
Contact List
| Marie Neu | |
|---|---|
| contact affiliation | Abbvie Deutschland GmbH & Co-KG |
| contact email | marie.neu@abbvie.com |
| lab head | |
| Marie Neu | |
| contact affiliation | Abbvie Deutschland Gmbh& Co KG |
| contact email | marie.neu@abbvie.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000096965/ |
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