PXD060166 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | AtNHR2A and AtNHR2B: two players of the secretory pathway functioning in plant immunity |
| Description | AtNHR2A and AtNHR2B were identified in Arabidopsis thaliana as two immune proteins given the susceptibility of the single mutants Atnhr2a and Atnhr2b to Pseudomonas syringae pv. tabaci (Pstab), a pathogen that does not infect wild-type Arabidopsis plants. Notably, the double mutant Atnhr2b Atnhr2a exhibited even greater susceptibility to Pstab than either single mutant, indicating that AtNHR2A and AtNHR2B function together in plant immunity. The subcellular localization of AtNHR2A and AtNHR2B to endomembrane compartments and their interaction with proteins known to be secreted led us to hypothesize that these two proteins participate in immune-related secretory processes. To investigate this, we isolated apoplastic fluid from the double mutant Atnhr2b Atnhr2a and the wild-type Col-0 for comparative proteome analysis. Using quantitative proteomics, we identified 52 Differentially Abundant Proteins (DAP) with N-terminal signal peptides in the apoplast of Atnhr2b Atnhr2a compared to Col-0, with ~50% showing lower abundance in Atnhr2b Atnhr2a, highlighting that Atnhr2b Atnhr2a is compromised in conventional protein secretion. Additionally, we developed an analytical pipeline to identify secreted proteins lacking signal peptides significantly enriched in the apoplast, revealing 59 previously unknown proteins whose secretion is dependent on AtNHR2A and AtNHR2B through a non-conventional secretory pathway. The differences in protein abundance within the apoplastic compartment provided compelling evidence that AtNHR2A and AtNHR2B are key mediators of the secretory pathway involved in plant immune response. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-30 |
| AnnouncementXML | Submission_2026-07-30_12:43:37.169.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sophe Alvarez |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; |
| ModificationList | monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-24 10:17:27 | ID requested | |
| ⏵ 1 | 2026-07-30 12:43:37 | announced | |
Publication List
Keyword List
| submitter keyword: Arabidopsis, proteomics,immunity, apoplastic |
Contact List
| Sophie Alvarez |
|---|
| contact affiliation | Proteomics & Metabolomics Facility, University of Nebraska-Lincoln |
| contact email | salvarez@unl.edu |
| lab head | |
| Sophe Alvarez |
|---|
| contact affiliation | University of Nebraska - Lincoln |
| contact email | salvarez@unl.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060166
- Label: PRIDE project
- Name: AtNHR2A and AtNHR2B: two players of the secretory pathway functioning in plant immunity
