PXD060153-1

PXD060153 is an original dataset announced via ProteomeXchange.

Title	Aggressive Gastric Cancer Overexpresses Trimmed N-Glycans and Exhibits Distinct Glycoproteome Signatures Predictive of Poor Prognosis
Description	Gastric cancer poses a significant threat at advanced stages, with the identification of molecular signatures for prognosis and targeted treatment remaining crucial yet challenging. This study investigates the under-characterized gastric cancer glycocalyx, revealing glycan signatures linked to cancer aggressiveness through high-throughput N-glycome analysis. Our findings show that aggressive tumors with poorer prognoses are enriched with trimmed paucimannosidic N-glycans. Lectin immunoblotting confirmed the disease-specific nature of these glycans, showing increased expression with cancer progression. Furthermore, TCGA analysis of over 400 cases revealed a correlation between key glycosyltransferase expression, associated with paucimannose biosynthesis, and poor prognosis, providing a potential molecular basis for these observations. Paucimannose N-glycans were predominantly found in intracellular ribosomal proteins challenging classical links with the plasma membrane proteome, though notable membrane proteins like CEACAM6 and MMP9 were identified. MMP9 demonstrated limited expression in healthy tissues, increasing with disease stage and grade. Tumors expressing MMP9 and paucimannosidic glycans also exhibited the worst prognosis. This work advances our understanding of gastric cancer glycome, highlighting the clinical context for paucimannosidic N-glycans and its underlying glycoproteome, providing potential targets for more precise therapeutic approaches.
HostingRepository	PRIDE
AnnounceDate	2025-10-13
AnnouncementXML	Submission_2025-10-12_17:50:12.402.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dylan Ferreira
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-24 06:03:44	ID requested
⏵ 1	2025-10-12 17:50:13	announced

Ferreira D, Marinho-Santos B, Relvas-Santos M, Orr B, Brand, ã, o A, Afonso LP, Santos LL, Ferreira JA, -glycans define aggressive gastric cancer and predict clinical outcomes. Theranostics, 15(18):9375-9398(2025) [pubmed]

10.7150/thno.111670;

submitter keyword: Cancer Glycobiology, Glycoproteome, Gastric Cancer, Glycome, Precision Oncology

José Alexandre Ferreira
contact affiliation	Research Center of IPO-Porto (CI-IPOP)/ RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO-Porto) / Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC Raquel Seruca) / GlycoMatters Biotech
contact email	jose.a.ferreira@ipoporto.min-saude.pt
lab head
Dylan Ferreira
contact affiliation	Research Center of Portuguese Oncology Institute of Porto
contact email	i38795@ipoporto.min-saude.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060153
     1. Label: PRIDE project
     2. Name: Aggressive Gastric Cancer Overexpresses Trimmed N-Glycans and Exhibits Distinct Glycoproteome Signatures Predictive of Poor Prognosis