PXD060148-1

PXD060148 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of commercial human primary mesangial cell, implications for experimental design.
Description	Research on human glomerular mesangial cells (MCs) is mainly performed in vitro on primary MCs. Commercially available MCs originate from different donors and thus, because of their origin, the outcome of the experiments could potentially be affected. We have compared two commercially available primary human MCs (HMCv1 and HMCv2) using proteomics. Baseline differences and differences after treatment with a known proliferation factor (PDGF-B) were investigated.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_17:06:42.847.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evelin Berger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-24 05:29:45	ID requested
⏵ 1	2025-10-05 17:06:43	announced

10.1186/s12882-025-04444-1;

Johansson A, Narasimhan G, Keuenhof K, Boi R, Ebefors K, Comparative analysis of commercial human primary mesangial cell, implications for experimental design. BMC Nephrol, 26(1):539(2025) [pubmed]

submitter keyword: PDGF-BB Primary human mesangial cells mesangium glomerulus, quantitative proteomics

Carina Sihlbom Wallem
contact affiliation	Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Sweden
contact email	carina.sihlbom@gu.se
lab head
Evelin Berger
contact affiliation	Proteomics Core Facility, University of Gothenburg
contact email	evelin.berger@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD060148

PRIDE project URI

• PRIDE
  1. PXD060148
     1. Label: PRIDE project
     2. Name: Comparative analysis of commercial human primary mesangial cell, implications for experimental design.