PXD060142-1

PXD060142 is an original dataset announced via ProteomeXchange.

Title	Metabolic dependency of ribosome heterogeneity in the human malaria parasite
Description	To infect new hosts, the human malaria parasite Plasmodium falciparum needs to transmit to a mosquito vector. One of the most compelling adaptations to the two different host environments is the transcription of divergent ribosomal RNA genes that are transcribed from distinct genomic loci. Here we show that the silencing of mosquito-stage specific rRNA transcription is coupled to the level of aerobic glycolysis, the parasites premier metabolic pathway in the human host. We show that NAD+ levels originating from lactate fermentation are translated on a molecular level by the histone deacetylase Sir2a. By comprehensively characterizing histone epigenetic modifications in Sir2a-KO and wild-type cells coupled with quantitative chromatin immunoprecipitation, we show that the parasite metabolic state is linked to a functional transcriptional output.
HostingRepository	PRIDE
AnnounceDate	2025-11-25
AnnouncementXML	Submission_2025-11-25_03:14:42.387.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanessa Masson
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: NEWT:5833;
ModificationList	monomethylated residue; trimethylated residue; acetylated residue; dimethylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-24 02:31:24	ID requested
⏵ 1	2025-11-25 03:14:43	announced

Dataset with its publication pending

submitter keyword: Sir2a-KO, Histone PTM,malaria, ribosomal DNA, Plasmodium falciparum

Damarys Loew
contact affiliation	Head of the Curie Institute Mass Spectrometry Platform
contact email	damarys.loew@curie.fr
lab head
Vanessa Masson
contact affiliation	Institut Curie - Centre de Recherche
contact email	vanessa.masson@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD060142

PRIDE project URI

• PRIDE
  1. PXD060142
     1. Label: PRIDE project
     2. Name: Metabolic dependency of ribosome heterogeneity in the human malaria parasite