PXD060122-1

PXD060122 is an original dataset announced via ProteomeXchange.

Title	Head and neck squamous cell carcinoma-derived small extracellular vesicles mediate Ca²⁺-dependent platelet activation and aggregation through tissue factor
Description	Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy, characterized by poor clinical outcomes, primarily driven by high rate of locoregional recurrence and metastasis. Extensive heterogeneity among the tumor cells as well as modulation of a highly immunosuppressive tumor microenvironment shape cancer progression. Shedding of small extracellular vesicles (sEVs) derived from tumor cells is a critical mediator of the disease initiating horizontal transfer of tumor components into platelets. HNSCC-derived sEVs isolated from HNSCC cell lines (SAS, UD-SCC 5) using Size exclusion chromatography and characterized via Flow cytometry, Electron microscopy, Nanoparticle tracking analysis and Western blotting, were used to induce platelet activation and aggregation, measured by aggregometry, flow cytometry, as well as the release of chemokines and ATP, which were quantified using Enzyme-linked immunosorbent assays (ELISA). Mechanistic investigations included inhibitor assays, thrombin activity measurements, and proteomic analyses. Unexpectedly we can show that sEVs do not activate PLTs through the FcγRIIa–IgG axis but platelet activation and aggregation is induced in a calcium-dependent manner, primarily mediated by sEV-associated Tissue factor. Proteomic analysis confirmed the presence of Tissue factor in these vesicles, implicating its involvement in initiating the coagulation cascade, that leads to platelet activation and aggregation.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_06:57:49.436.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Mergner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-23 09:42:57	ID requested
⏵ 1	2025-05-07 06:57:50	announced

Weiser T, Hoch CC, Petry J, Shoykhet M, Schmidl B, Yazdi M, Hachani K, Mergner J, Theodoraki MN, Azimzadeh O, Multhoff G, Bashiri Dezfouli A, Wollenberg B, -dependent platelet activation and aggregation through tissue factor. Cell Commun Signal, 23(1):210(2025) [pubmed]

10.1186/s12964-025-02215-x;

submitter keyword: Small extracellular vesicles

Tissue factor,Coagulation cascade, Head and neck squamous cell carcinoma

Platelet activation, Cancer-associated thrombosis

Julia Mergner
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry at Klinikum rechts der Isar (BayBioMS@MRI), TUM School of Medicine and Health, Technical University of Munich (TUM), 81675 München, Germany
contact email	julia.mergner@tum.de
lab head
Julia Mergner
contact affiliation	Chair of Proteomics and Bioanalytics, TU Munich
contact email	julia.mergner@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060122

PRIDE project URI

• PRIDE
  1. PXD060122
     1. Label: PRIDE project
     2. Name: Head and neck squamous cell carcinoma-derived small extracellular vesicles mediate Ca²⁺-dependent platelet activation and aggregation through tissue factor