PXD060118-1

PXD060118 is an original dataset announced via ProteomeXchange.

Title	Latent infection of an active giant endogenous virus in a unicellular green alga
Description	Latency is a common strategy in a wide range of viral lineages, but its prevalence in giant viruses remains unknown. Here we describe the activity and viral production from a 617 kbp integrated giant viral element in the model green alga Chlamydomonas reinhardtii. We resolve the integrated viral region using long-read sequencing and show that viral particles are produced and released in otherwise healthy cultures. A diverse array of viral-encoded selfish genetic elements are expressed during GEVE reactivation and produce proteins that are packaged in virions. In addition, we show that field isolates of Chlamydomonas sp. harbor latent giant viruses related to the C. reinhardtii GEVE that exhibit similar infection dynamics, demonstrating that giant virus latency is prevalent in natural host communities. Our work reports the largest temperate virus documented to date and the first active GEVE identified in a unicellular eukaryote, substantially expanding the known limits of viral latency.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-15_16:13:16.773.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060118
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Keith Ray
SpeciesList	scientific name: Nucleocytoviricota; NCBI TaxID: 2732007;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-23 09:00:30	ID requested
⏵ 1	2025-06-15 16:13:17	announced

10.6019/PXD060118;

Erazo-Garcia MP, Sheyn U, Barth ZK, Craig RJ, Wessman P, Jivaji AM, Ray WK, Svensson-Coelho M, Cornwallis CK, Rengefors K, Brussaard CPD, Moniruzzaman M, Aylward FO, Cryptic infection of a giant virus in a unicellular green alga. Science, 388(6748):eads6303(2025) [pubmed]

10.1126/science.ads6303;

submitter keyword: Chlamydomonas reinhardtii,Giant Endogenous Viral Elements (GEVE)

Dr. Frank O. Aylward
contact affiliation	Department of Biological Sciences and the Center for Emerging, Zoonotic, and Arthropod-Borne Pathogens, Virginia Tech, Blacksburg, VA
contact email	faylward@vt.edu
lab head
Keith Ray
contact affiliation	Virginia Tech
contact email	wkray@vt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD060118

PRIDE project URI

• PRIDE
  1. PXD060118
     1. Label: PRIDE project
     2. Name: Latent infection of an active giant endogenous virus in a unicellular green alga