PXD060112-1

PXD060112 is an original dataset announced via ProteomeXchange.

Title	Dexter Energy Transfer Photocatalysis Enables Covalent Label-ing and Profiling of Amyloid Plagues in Alzheimer’s Disease Tissue
Description	Amyloid plaque is a pathological hallmark in Alzheimer's disease (AD) brain with their composition associated with dis-ease etiology. Here, we report a photocatalyst, namely AmyCAT, that selectively binds to amyloid fibrils in AD brain tis-sue and undergoes Dexter energy transfer (DET) to catalyze carbene production for amyloid proximity labeling. First, we design the AmyCAT photocatalyst to generally bind different types of amyloids. Second, we energetically match the DET effect in a 12 × 4 photocatalysts-substrates array to activate diazo substrate into reactive carbene by visible green light (530~545 nm). Further, we demonstrate that the optimal AmyCAT probe catalyzes pan-amyloid protein labeling with 30-fold selectivity over folded counterparts. Mechanistic studies confirm the photocatalytic labeling is indeed via DET pro-cess, which tailors the proximity effect for spatially confined amyloid labeling. Finally, we employ the AmyCAT photo-catalyst to label, enrich and profile amyloid plagues in AD brain tissue, revealing key proteins and pathways associated with AD pathological deposition and etiology.
HostingRepository	PRIDE
AnnounceDate	2025-01-23
AnnouncementXML	Submission_2025-01-23_06:13:33.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Sun
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-23 06:07:20	ID requested
⏵ 1	2025-01-23 06:13:33	announced

Dataset with its publication pending

submitter keyword: Dexter energy transfer,protein labeling, amyloid, carbene, chemoproteomics, photocatalysis

Yu Liu
contact affiliation	State Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
contact email	liuyu@dicp.ac.cn
lab head
Rui Sun
contact affiliation	Dalian institute of chemical physics
contact email	sunrui@dicp.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD060112

PRIDE project URI

• PRIDE
  1. PXD060112
     1. Label: PRIDE project
     2. Name: Dexter Energy Transfer Photocatalysis Enables Covalent Label-ing and Profiling of Amyloid Plagues in Alzheimer’s Disease Tissue