PXD060087-1

PXD060087 is an original dataset announced via ProteomeXchange.

Title	Improved wound healing by dual inhibition of miR-146a-5p and miR-29a-3p supports a network action of dysregulated miRNAs in diabetic skin
Description	MiRNA-146a-5p, and -29-3p are repeatedly reported to be dysregulated in wound healing, however, not much is known about their roles in in the immune inflammatory responses and skin regeneration within cellular networks. We tested our hypothesis that suppressing the two over-expressed miRNAs would address wound healing impairments, using two distinct models of wound healing: Immortalised keratinocytes (HaCaT cells) transfected with LNA (locked nucleic acid)-spiked fully phosphorothioate modified antisense miR-146a-5p (5’-TGgaauucAgTuCucA-3’), miR-29a-3p (5’-AuuTcaGauGguGcuA-3’) oligos (LNA bases are written in capital letters while 2′-O-methyl RNA bases in small letters) and a scramble (Scr) control oligo (5’-CaaTagGguCaaGauT-3’) individually or in combination, and STZ induced type 1 diabetes animal model of wound healing. We observed significant corrections of dysfunctional cell migration (p<0.01 on day 8; and p<0.05 on day 9), decreased inflammation at day 3 (p<0.01) and day 10 (p<0.0001), reduction of excessive production of reactive oxygen species (p<0.01) 3 days post-wounding while increasing the density of blood vessel networks on day 3 (p<0.01) and day 10 (p<0.0001). This was consistent with cytoskeletal rearrangements and collagen alterations observed with proteome profiling. Our data suggest that synergetic potential of dual inhibition of the two miRNAs is a superior approach compared to individual miRNA inhibition, and a potential treatment for a multitude of wound healing impairments observed in diabetic wounds.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-14_20:49:38.947.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcus Nielsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-22 14:46:08	ID requested
⏵ 1	2025-12-14 20:49:39	announced

Petkovic M, Leal EC, S, ø, rensen AE, J, ø, rgensen PT, Wengel JT, Jersie-Christensen RR, Troelsen JT, Carvalho E, Dalgaard LT, Improved wound healing by dual inhibition of miR-146a-5p and miR-29a-3p supports a network action of dysregulated miRNAs in diabetic skin. Diabetologia, 69(1):214-229(2026) [pubmed]

10.1007/s00125-025-06522-3;

submitter keyword: Human, skin, mouse, diabetes

Louise Torp Dalgaard
contact affiliation	Roskilde University, Denmark Institute for Science and Environment Molecular and Medical Biology
contact email	ltd@ruc.dk
lab head
Marcus Nielsen
contact affiliation	Roskilde university
contact email	marcusn@ruc.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD060087

PRIDE project URI

• PRIDE
  1. PXD060087
     1. Label: PRIDE project
     2. Name: Improved wound healing by dual inhibition of miR-146a-5p and miR-29a-3p supports a network action of dysregulated miRNAs in diabetic skin