PXD060043-1
PXD060043 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of sarkosyl-insoluble proteins that accumulate in the brains of aged mice. |
| Description | Five young (2-month-old) and five aged (24-month-old) mouse brains were bisected along the midline to separate the right and left hemispheres, then snap-frozen in liquid nitrogen. The frozen tissues were pulverized using a Multi-Beads Shocker (Yasui Kikai) and stored at -150°C until further use. The brain powder was homogenized in sarkosyl buffer (1% sarkosyl, 10 µg/mL RNase, 1 mM PMSF, 1 mM EDTA in PBS) using a Potter-Elvehjem homogenizer at 800 rpm. After a 10-minute incubation on ice, the homogenate was centrifuged at 15,000 g for 5 minutes at 4°C to separate the supernatant and pellet (P1). The supernatant was centrifuged again at 15,000 g for 5 minutes at 4°C, followed by a third centrifugation at 15,000 g for 15 minutes at 4°C to obtain the final supernatant (S1) and a pellet. S1 was then subjected to ultracentrifugation at 100,000 g for 1 hour at 4°C, resulting in a supernatant (S2) and a pellet. This pellet was resuspended in sarkosyl buffer using a vortex mixer and centrifuged again at 100,000 g for 1 hour at 4°C. The supernatant was discarded, and the final pellet (P2), representing the sarkosyl-insoluble fraction, was retained. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-08-04 |
| AnnouncementXML | Submission_2025-08-03_08:00:27.492.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD060043 |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | SHirayama |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | L-cysteine methyl disulfide; L-methionine sulfoxide |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-01-21 21:11:31 | ID requested | |
| ⏵ 1 | 2025-08-03 08:00:27 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Aging, Protein aggregation |
Contact List
| Shigeo Murata | |
|---|---|
| lab head | |
| SHirayama | |
| contact affiliation | Hamamatsu University School of Medicine |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003565/ |
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