PXD060041-1

PXD060041 is an original dataset announced via ProteomeXchange.

Title	Identification of the epitope recognized by a specific VHH raised against the Th17 antigen (AID45212)
Description	Segmented filamentous bacteria (SFB) are ubiquitous intestinal commensals that promote immune system development and pathogen protection. The unicellular teardrop-shaped SFB, called intracellular offsprings (IOs), attach to ileal epithelial cells via their unique tip structure and outgrow into filaments. To obtain a more detailed view of the SFB surface, particularly at the tip structure, we used a combination of cryo-EM and cryo-ET to characterize the surface structures present in IOs and filaments from mouse- and rat-SFB. HDX-MS was used to identified the epitope recognized by a specific VHH raised against the Th17 antigen (AID45212) located at the SFB surface.
HostingRepository	PRIDE
AnnounceDate	2025-10-21
AnnouncementXML	Submission_2025-10-21_08:19:20.844.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sébastien Brier
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-21 19:26:43	ID requested
⏵ 1	2025-10-21 08:19:21	announced

Dataset with its publication pending

ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS)

submitter keyword: Segmented Filamenteous Bacteria (SFB), Hydrogen/Deuterium eXchange mass spectrometry (HDX-MS),Epitope mapping, Th17 antigen (AID45212)

Sebastien BRIER
contact affiliation	Institut Pasteur, Université Paris Cité, CNRS UMR3528, Biological NMR and HDX-MS Technological Platform, 75015, Paris, France.
contact email	sebastien.brier@pasteur.fr
lab head
Sébastien Brier
contact affiliation	Institut Pasteur, Université Paris Cité, BioNMR and HDX-MS facility, Center for Technological Resources and Research, Chemistry and Structural Biology Department, UMR CNRS 3528, F-75015 Paris, France
contact email	sebastien.brier@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD060041

PRIDE project URI

• PRIDE
  1. PXD060041
     1. Label: PRIDE project
     2. Name: Identification of the epitope recognized by a specific VHH raised against the Th17 antigen (AID45212)