PXD060036-1

PXD060036 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of T24 bladder cancer cell nuclear extracts after tailored chemical interventions
Description	Bladder cells are constantly exposed to multiple chemical stressors stemming from urine, including xenobiotics and bioactive metabolites. Even if not completely understood at the molecular level, a significant adaptability to these challenges is a prerequisite for bladder cells and enables the maintenance of physiological function. Here untargeted profiling was applied to grasp changes in bladder cells proteome upon application of tailored chemical interventions. To focus on regulatory events observable in the nuclear and peri-nuclear compartments, nuclear extracts were generated after incubation with different molecules enabling regulation of oxidative stress (1 µM MitoTEMPOL, 500 µM H2O2), autophagy (10 nM Bafilomycin A1), cytoskeletal integrity (100 µM CK666), and metabolism (1 mM 2-desoxyglucose). T24 cells were incubated with the respective treatments for 24 hours, after which nuclear proteins were isolated, digested and analyzed using a timsTOF Pro mass spectrometer (Bruker). Identification as well as label-free quantification (LFQ) of proteins and statistical analyses were performed using publicly available software package MaxQuant 1.6.17.0.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_20:35:34.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-21 15:46:52	ID requested
⏵ 1	2025-08-03 20:35:35	announced

Jobst M, Crudo F, Marko D, Bileck A, Meier-Menches SM, Gerner C, Del Favero G, Retrograde rearrangement of mitochondria correlates with nuclear deformation and genotoxic damage. iScience, 28(8):112955(2025) [pubmed]

10.1016/j.isci.2025.112955;

submitter keyword: genotoxicity, mechanotransduction, T24 cell line, mitochondria, DNA damage,oxidative stress, mitochondrial retrograde response, nuclear deformation

Christopher Gerner
contact affiliation	University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060036
     1. Label: PRIDE project
     2. Name: Proteome analysis of T24 bladder cancer cell nuclear extracts after tailored chemical interventions