PXD060031-1

PXD060031 is an original dataset announced via ProteomeXchange.

Title	Identification of KIF20A binding partners using IP-mass spectrometry analysis
Description	Breast cancer stem cells (BCSCs), known to fuel TNBC malignancy, are now being exploited as the vulnerability for TNBC treatment. In this study, we dissected the transcriptome of BCSCs and identified Kinesin Family Member 20A (KIF20A) as an essential gene for BCSC survival and TNBC tumorigenesis. To understand the potential mechanism, we infected MDA-MB-231 cells with either control or V5-tagged KIF20A followed by V5 antibody pull down and mass spectrometry to identify KIF20A interaction proteins that may mediate its effect on gene regulation and TNBC cancer stem cell phenotype.
HostingRepository	PRIDE
AnnounceDate	2025-09-30
AnnouncementXML	Submission_2025-09-30_11:19:01.633.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ling Xie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-21 12:36:14	ID requested
⏵ 1	2025-09-30 11:19:02	announced

Dataset with its publication pending

submitter keyword: KIF20A, IP-mass spectrometry,MDA-MB-231

Qing Zhang
contact affiliation	UT Southwestern Medical Center
contact email	Qing.Zhang@utsouthwestern.edu
lab head
Ling Xie
contact affiliation	UNC at Chapel Hill
contact email	xiel@email.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD060031

PRIDE project URI

• PRIDE
  1. PXD060031
     1. Label: PRIDE project
     2. Name: Identification of KIF20A binding partners using IP-mass spectrometry analysis