PXD060025-1

PXD060025 is an original dataset announced via ProteomeXchange.

Title	For euchromatic repeats RID is required for both Repeat Induced Point mutation and nucleation of a novel transitional heterochromatic state
Description	Gene inactivation via heterochromatization is important for genome integrity and prevention of unwanted transcripts with dysregulation in cancer. Repeat sequences are subject to heterochromatin inactivation and, in Neurospora, Repeat-Induced-Point mutation (RIP). The initiating factors behind both are poorly understood. We resolve the paradoxical observation that newly introduced Repeat-Linker-Repeat (RLR) constructs require RID alone for RIP, while genomic repeats are RIPed in the absence of RID, showing that eu- and hetero- chromatic repeats are handled differently, the latter requiring DIM-2 as well. The differences between mechanisms associated with older and newer duplicates caution against extrapolation from mechanisms inferred from model experimental systems to all genomically relevant processes. Additionally, while chromatin status affects RIP, RID we also show via lexA tethering is the nucleation centre for a transition from eu- to hetero- chromatin, in an HDA-1 dependent fashion. Constitutive heterochromatin by contrast is HDA1 independent and depends on HDA-1 paralogs. RID is thus a dual function initiator of both RIP and the transition to heterochromatin.
HostingRepository	PRIDE
AnnounceDate	2025-03-28
AnnouncementXML	Submission_2025-03-28_05:29:19.008.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ju Huang
SpeciesList	scientific name: Neurospora crassa OR74A; NCBI TaxID: 367110;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-21 10:00:22	ID requested
⏵ 1	2025-03-28 05:29:19	announced

10.1093/NAR/GKAF263;

submitter keyword: Repeat Induced Point mutation, heterochromatin, Neurospora

Ju Huang
contact affiliation	Nanjing Agricultural University
contact email	ponyhuang@njau.edu.cn
lab head
Ju Huang
contact affiliation	Nanjing Agricultural University
contact email	ponyhuang@njau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD060025

PRIDE project URI

• PRIDE
  1. PXD060025
     1. Label: PRIDE project
     2. Name: For euchromatic repeats RID is required for both Repeat Induced Point mutation and nucleation of a novel transitional heterochromatic state