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PXD060000-1

PXD060000 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Optimising HCD fragmentation energy for TMT-labelled intact glycopeptide samples
Description	It was crucial to choose the appropriate dissociation method and collision energy for large-scale glycoproteomics studies. HCD has been successfully applied to large-scale glycoproteomics experiments31. We know that TMT-labelled glycopeptides require higher collision energies for identification and quantitative accuracy because glycosidic bonds can absorb part of the energy32. Therefore, we systematically evaluated the performance of different HCD collision energies in characterizing TMT-labelled intact N-glycopeptides based on the StrucGP high HCD energy identification of peptides, and low HCD energy identification of glycans by fragmentation strategy.
HostingRepository	iProX
AnnounceDate	2025-01-20
AnnouncementXML	Submission_2025-09-27_07:43:04.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhida Zhang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-20 19:26:22	ID requested
⏵ 1	2025-09-27 07:43:05	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: TMT_labeling, HCD energy

Contact List

Shisheng Sun
contact affiliation	Northwest University
contact email	suns@nwu.edu.cn
lab head
Zhida Zhang
contact affiliation	Northwest University
contact email	zhida0717@163.com
dataset submitter

Full Dataset Link List

iProX dataset URI